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Radek Šindelka, PhD

Radek Sindelka

Radek Sindelka, PhD

Senior scientist

Areas of expertise

  • Gene expression profiling
  • Single cell analysis
  • Developmental biology
  • Genetics
  • Education
  • Teaching


I am a researcher in the Laboratory of Gene Expression leading a group of young students who are mainly working on projects focused on a combination of methods such as RT-qPCR, RNA-Seq, single cell analysis and functional assays using the interesting model organism Xenopus laevis. During my PhD I developed tools for spatial and temporal expression profiling in Xenopus eggs and embryos. Then I spent four years as a postdoc in Prof. Sive lab at Whitehead Institute, MIT in USA, where I studied the role of Kinin-kallikrein pathway during head development. After my return to Czech Republic, I have continued with projects focused on the role of nitric oxide (product of Kinin-kallikrein pathway) during development, wound healing and regeneration. I collaborate closely with Czech and foreign laboratories, which provide us unique biological models and modern techniques. Out of the lab, I spend my time with family and friends.

Selected Publications

  1. The real-time polymerase chain reaction. Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonák J, Lind K, Sindelka R, Sjöback R, Sjögreen B, Strömbom L, Ståhlberg A, Zoric N. Mol Aspects Med. 2006. 27(2-3):95-125.
  2. Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte. Sindelka R, Jonák J, Hands R, Bustin SA, Kubista M. Nucleic Acids Res. 2008. 36(2):387-92.
  3. The extreme anterior domain is an essential craniofacial organizer acting through Kinin-Kallikrein signaling. Jacox L, Sindelka R, Chen J, Rothman A, Dickinson A, Sive H. Cell Rep. 2014. 8(2):596-609. doi: 10.1016/j.celrep.2014.06.026.
  4. Single blastomere expression profiling of Xenopus laevis embryos of 8 to 32-cells reveals developmental asymmetry. Flachsova M, Sindelka R, Kubista M. Sci Rep. 2013;3:2278. doi: 10.1038/srep02278.
  5. The role of nitric oxide during embryonic epidermis development of Xenopus laevis. Tomankova S, Abaffy P, Sindelka R. Biol Open. 2017. 6(6):862-871. doi: 10.1242/bio.023739.