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Multicentric Evaluation of Circulating Plasma MicroRNA Extraction Technologies...

Kloten V, Neumann MHD, Di Pasquale F, Sprenger-Haussels M, Shaffer JM, Schlumpberger M, Herdean A, Betsou F, Ammerlaan W, Af Hällström T, Serkkola E, Forsman T, Lianidou E, Sjöback R, Kubista M, Bender S, Lampignano R, Krahn T, Schlange T; CANCER-ID consortium. Clin Chem. 2019 Jun 24. pii: clinchem.2019.303271. doi: 10.1373/clinchem.2019.303271.



Detection of Abundant Non-Haematopoietic Circulating Cancer-Related Cells in Patients...

Kumar, J.; Chudasama, D.; Roberts, C.; Kubista, M.; Sjöback, R.; Chatterjee, J.; Anikin, V.; Karteris, E.; Hall, M. Detection of Abundant Non-Haematopoietic Circulating Cancer-Related Cells in Patients with Advanced Epithelial Ovarian Cancer. Cells 2019, 8, 732.



The legacy of sexual ancestors in phenotypic variability, gene expression and homoeolog...

The legacy of sexual ancestors in phenotypic variability, gene expression and homoeolog regulation of asexual hybrids and polyploids. Bartoš O, Röslein J, Kotusz J, Paces J, Pekárik L, Petrtýl M, Halačka K, Štefková Kašparová E, Mendel J, Boroń A, Juchno D, Leska A, Jablonska O, Benes V, Šídová M, Janko K. Mol Biol Evol. 2019 May 11. pii: msz114. doi: 10.1093/molbev/msz114.



Two-tailed RT-qPCR panel for quality control of circulating microRNA studies.

Androvic P, Romanyuk N, Urdzikova-Machova L, Rohlova E, Kubista M, Valihrach L. Two-tailed RT-qPCR panel for quality control of circulating microRNA studies. Sci Rep. 2019 Mar 12;9(1):4255. doi: 10.1038/s41598-019-40513-w.



Metformin Increases Proliferative Activity and Viability of Multipotent Stromal Stem...

Metformin Increases Proliferative Activity and Viability of Multipotent Stromal Stem Cells Isolated from Adipose Tissue Derived from Horses with Equine Metabolic Syndrome. Smieszek A, Kornicka K, Szłapka-Kosarzewska J, Androvic P, Valihrach L, Langerova L, Rohlova E, Kubista M, Marycz K. Cells. 2019; 8(2):80.



Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores...

Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells. Bajzikova M, Kovarova J, Coelho AR, Boukalova S, Oh S, Rohlenova K, Svec D, Hubackova S ... Kolar F, Kubista M, Truksa J, Dvorakova-Hortova K, Pacak K, Gurlich R, Stocker R, Zhou Y, Berridge MV, Park S, Dong L, Rohlena J, Neuzil J. Cell Metab. 2018 Nov 9. pii: S1550-4131(18)30646-6. doi: 10.1016/j.cmet.2018.10.014.



The contribution of TRPV4 channels to astrocyte volume regulation and brain edema...

The contribution of TRPV4 channels to astrocyte volume regulation and brain edema formation. Pivonkova H, Hermanova Z, Kirdajova D, Awadova T, Malinsky J, Valihrach L, Zucha D, Kubista M, Galisova A, Jirak D, Anderova M. Neuroscience. 2018 Oct 24. pii: S0306-4522(18)30691-2. doi: 10.1016/j.neuroscience.2018.10.028.



Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons.

Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons. Andersson D, Svec D, Pedersen C, Henriksen JR, Ståhlberg A. Int J Mol Sci. 2018 Oct 16;19(10). pii: E3185. doi: 10.3390/ijms19103185.



Circulating tumor cells mirror bone metastatic phenotype in prostate cancer.

Josefsson A, Larsson K, Månsson M, Björkman J, Rohlova E, Åhs D, Brisby H, Damber JE, Welén K. Oncotarget. Circulating tumor cells mirror bone metastatic phenotype in prostate cancer. 2018 Jun 29;9(50):29403-29413. doi: 10.18632/oncotarget.25634. eCollection 2018 Jun 29.



Adverse effects of Hif1a mutation and maternal diabetes on the offspring heart

Cerychova R, Bohuslavova R, Papousek F, Sedmera D, Abaffy P, Benes V, Kolar F, Pavlinkova G. Adverse effects of Hif1a mutation and maternal diabetes on the offspring heart. Cardiovasc Diabetol. 2018 May 12;17(1):68. doi: 10.1186/s12933-018-0713-0.



Alternative assembly of respiratory complex II connects energy stress to metabolic...

Bezawork-Geleta A, Wen H, Dong L, Yan B, Vider J, Boukalova S, Krobova L, Vanova K, Zobalova R, Sobol M, Hozak P, Novais SM, Caisova V, Abaffy P, Naraine R, Pang Y, Zaw T, Zhang P, Sindelka R, Kubista M, Zuryn S, Molloy MP, Berridge MV, Pacak K, Rohlena J, Park S, Neuzil J. Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints. Nat Commun. 2018 Jun 7;9(1):2221. doi: 10.1038/s41467-018-04603-z.



Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg ...

Sindelka, R., Abaffy, P., Qu, Y., Tomankova, S., Sidova, M., Naraine, R., Kolar, M., Peuchen, E., Sun, L., Dovichi, N., Kubista, M. Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg and their impact on the developmental plan. Scientific Reports, 8: 8315, 2018. doi: 10.1038/s41598-018-26592-1. ISSN: 2045-2322.



Gene Expression Analysis of Immunomagnetically Enriched Circulating Tumor Cell Fraction...

Skerenova, M., Mikulova, V., Capoun, O., Svec, D., Kolostova, K., Soukup, V., Honova, H.,Hanus, T., Zima, T. Gene Expression Analysis of Immunomagnetically Enriched Circulating Tumor Cell Fraction in Castration-Resistant Prostate Cancer. Molecular Diagnosis & Therapy, 22 (3): 381–390, 2018 . doi: 10.1007/s40291-018-0333-0. ISSN: 1177-1062



Identification of inhibitors regulating cell proliferation and FUS-DDIT3 expression ...

Svec, D., Dolatabadi, S., Thomsen, C., Cordes, N., Shannon, M., Fitzpatrick, P., Landberg, G., Aman, P., Stahlberg, A. Identification of inhibitors regulating cell proliferation and FUS-DDIT3 expression in myxoid liposarcoma using combined DNA, mRNA, and protein analyses. Laboratory Investigation 2018 Mar 27. doi: 10.1038/s41374-018-0046-3.



The secrets of the cell

Kubista, M., Dreyer-Lamm, J., Stahlberg, A. The secrets of the cell. Molecular Aspects of Medicine, 59: 1-4, 2018. doi: 10.1016/j.mam.2017.08.004. ISSN: 0098-2997.



Comparison of oocyte mRNA localization patterns in sterlet Acipenser ruthenus and ...

Pochnerniaieva, K., Sidova, M., Havelka, M., Saito, M., Psenicka, M., Sindelka, R., Kaspar, V. Comparison of oocyte mRNA localization patterns in sterlet Acipenser ruthenus and African clawed frog Xenopus laevis. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, [ahead of print], 2018. doi: 10.1002/jez.b.22802. ISSN 1552-5015.



Technical aspects and recommendations for single-cell qPCR

Stahlberg, A., Kubista, M. Technical aspects and recommendations for single-cell qPCR. Molecular Aspects of Medicine, 59: 28-35, 2018. doi: 10.1016/j.mam.2017.07.004. ISSN: 0098-2997.



Platforms for Single-Cell Collection and Analysis

Valihrach, L., Androvic, P., Kubista, M. Platforms for Single-Cell Collection and Analysis. International Journal of Molecular Sciences, 19(3): 807, 2018. doi: 10.3390/ijms19030807. ISSN 1422-0067.



A single-cell analysis reveals multiple roles of oligodendroglial lineage cells during ...

Valny, M., Honsa, P., Waloschkova, E., Matuskova, H., Kriska, J., Kirdajova, D., Androvic, P., Valihrach, L., Kubista, M., Anderova, M. A single-cell analysis reveals multiple roles of oligodendroglial lineage cells during post-ischemic regeneration. Glia, 66(5): 1068-1081, 2018. doi: 10.1002/glia.23301. ISSN 1098-1136.



Human oocyte maturation in vitro is improved by co-culture with cumulus cells from ...

Virant-Klun, I., Bauer, C., Stahlberg, A., Kubista, M., Skutella, T. Human oocyte maturation in vitro is improved by co-culture with cumulus cells from mature oocytes. Reproductive BioMedicine Online, 36(5): 508-523, 2018. doi: 10.1016/j.rbmo.2018.01.011. ISSN 1472-6483.



Molecular characterization of circulating tumor cells from patients with metastatic ...

Aaltonen, K. E., Novosadova, V., Bendahl, P., Graffman, C., Larsson, A., Ryden, L. Molecular characterization of circulating tumor cells from patients with metastatic breast cancer reflects evolutionary changes in gene expression under the pressure of systemic therapy. Oncotarget, 8:45544-45565, 2017. doi: 10.18632/oncotarget.17271. eISSN 1949-2553.



Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification

Androvic, P., Valihrach, L., Elling, J., Sjoback, R., Kubista, M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Research, gkx588, 2017. doi: 10.1093/nar/gkx588. ISSN 1362-4962.



Gene Expression Signatures in Circulating Tumor Cells Correlate with Response to ...

Bredemeier, M., Edimiris, P., Mach, P., Kubista, M., Sjoback, R., Rohlova, E., Kolostova, K., Hauch, S., Aktas, B., Tewes, M., Kimmig, R., Kasimir-Bauer, S. Gene Expression Signatures in Circulating Tumor Cells Correlate with Response to Therapy in Metastatic Breast Cancer. Clinical Chemistry, 63(10): 1585-1593, 2017. doi: 10.1373/clinchem.2016.269605. ISSN 0009-9147.



Horizontal transfer of whole mitochondria restores tumorigenic potential in ...

Dong, L. F., Kovarova, J., Bajzikova, M., Bezawork-Geleta, A., Svec, D.,... Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells. eLife, 6:e22187, 2017. doi: 10.7554/eLife.22187. ISSN 2050-084X.



Methods to determine limit of detection and limit of quantification in quantitative ...

Forootan, A., Sjoback, R., Bjorkman, J., Sjogreen, B., Linz, L., Kubista, M. Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR). Biomolecular Detection and Quantification, [ahead of print], 2017. doi: 10.1016/j.bdq.2017.04.001. ISSN 2214-7535.



Asymmetric Localization and Distribution of Factors Determining Cell Fate During Early ...

Sindelka, R., Sidova, M., Abaffy, P., Kubista, M. Asymmetric Localization and Distribution of Factors Determining Cell Fate During Early Development of Xenopus laevis. Chapter in Asymmetric Cell Division in Development, Differentiation and Cancer, Springer International Publishing, ed. J.-P. Tassan, J. Z. Kubiak, Results and Problems in Cell Differentiation, 61: 229-241, 2017. doi: 10.1007/978-3-319-53150-2_10. ISBN 978-3-319-53149-6.



The role of nitric oxide during embryonic epidermis development of Xenopus laevis

Tomankova, S., Abaffy, P., Sindelka, R. The role of nitric oxide during embryonic epidermis development of Xenopus laevis. Biology Open, bio.023739, 2017. doi: 10.1242/bio.023739. ISSN 2046-6390.



Regulatory mechanisms, expression levels and proliferation effects of the FUS-DDIT3 ...

Aman, P., Dolatabadi, S., Svec, D., Jonasson, E., Safavi, S., Andersson, D., Grundevik, P., Thomsen, C., Stahlberg, A. Regulatory mechanisms, expression levels and proliferation effects of the FUS-DDIT3 fusion oncogene in liposarcoma. Journal of Pathology, 238(5): 689-699, 2016. doi: 10.1002/path.4700. ISSN 1096-9896.



Differential amplicons (ΔAmp) - a new molecular method to assess RNA integrity

Bjorkman, J., Svec, D., Lott, E., Kubista, M., Sjoback, R. Differential amplicons (ΔAmp) - a new molecular method to assess RNA integrity. Biomolecular Detection and Quantification, Special Issue: Advanced Molecular Diagnostics for Biomaker Discovery - Part II(6): 4-12, 2016. doi: 10.1016/j.bdq.2015.09.002. ISSN 2214-7535.



Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and ...

Conrad, S., Azizi, H., Hatami, M., Kubista, M., Bonin, M., Hennenlotter, J., Slevert, K.-D., Skutella, T. Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells. Stem Cells International, 2016: 8582526, 2016. doi: 10.1155/2016/8582526.