Publications

Human bone marrow stromal cells: the impact of anticoagulants on stem cell properties. Ferencakova M, Benova A, Raska I, Abaffy P, Sindelka R, Dzubanova M, Pospisilova E, Kolostova K, Cajka T, Paclik A, Zikan V, Tencerova M. Frontiers in Cell and Developmental Biology. 2023. 11. 10.3389/fcell.2023.1255823

NEUROD1 reinforces endocrine cell fate acquisition in pancreatic development. Bohuslavova R, Fabriciova V, Smolik O, Lebrón-Mora L, Abaffy P, Benesova S, Zucha D, Valihrach L, Berkova Z, Saudek F, Pavlinkova G. Nat Commun. 2023 Sep 9;14(1):5554. doi: 10.1038/s41467-023-41306-6.

Cortical glia in SOD1(G93A) mice are subtly affected by ALS-like pathology. Filipi T, Matusova Z, Abaffy P, Vanatko O, Tureckova J, Benesova S, Kubiskova M, Kirdajova D, Zahumensky J, Valihrach L, Anderova M.Sci Rep. 2023 Apr 21;13(1):6538. doi: 10.1038/s41598-023-33608-y.

Reactive astrogliosis in the era of single-cell transcriptomics. Matusova Z, Hol EM, Pekny M, Kubista M, Valihrach L. Front Cell Neurosci. 2023 Apr 20;17:1173200. doi: 10.3389/fncel.2023.1173200.

Dysregulation of hypoxia-inducible factor 1α in the sympathetic nervous system accelerates diabetic cardiomyopathy. Hrabalova P, Bohuslavova R, Matejkova K, Papousek F, Sedmera D, Abaffy P, Kolar F, Pavlinkova G. Cardiovasc Diabetol. 2023 Apr 18;22(1):88. doi: 10.1186/s12933-023-01824-5.

ISL1 controls pancreatic alpha cell fate and beta cell maturation. Bohuslavova R, Fabriciova V, Lebrón-Mora L, Malfatti J, Smolik O, Valihrach L, Benesova S, Zucha D, Berkova Z, Saudek F, Evans SM, Pavlinkova G. Cell Biosci. 2023 Mar 10;13(1):53. doi: 10.1186/s13578-023-01003-9.

Enhancement of zebrafish sperm production via a large body-sized surrogate with germ cell transplantation. Nayak R, Franěk R, Šindelka R, Pšenička M. Commun Biol. 2023 Apr 14;6(1):412. doi: 10.1038/s42003-023-04800-7.

Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action. Untergasser A, Hellemans J, Pfaffl MW, Ruijter JM, van den Hoff MJB, Dragomir MP, Adamoski D, Dias SMG, Reis RM, Ferracin M, Dias-Neto E, Marsh I, Kubista M, Fabbri M, Goel A, Slabý O, Knutsen E, Chen B, Negrini M, Mimori K, Pichler M, Papatriantafyllou M, Anfossi S, Schmittgen TD, Huggett J, Bustin S, Vandesompele J, Calin GA; HEROIC Consortium. Mol Oncol. 2023 Mar 14. doi: 10.1002/1878-0261.13418.

β-Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA-mRNA-miRNA regulatory associations. Data K, Marcinkowska K, Buś K, Valihrach L, Pawlak E, Śmieszek A.J Cell Mol Med. 2023 Mar 1. doi: 10.1111/jcmm.17694.

Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration. Fedorova V, Pospisilova V, Vanova T, Cerna KA, Abaffy P, Sedmik J, Raska J, Vochyanova S, Matusova Z, Houserova J, Valihrach L, Hodny Z, Bohaciakova D.Mol Oncol. 2023 Feb 6. doi: 10.1002/1878-0261.13389.

Mujahid Ali Shah, Effrosyni Fatira, Viktoriia Iegorova, Xuan Xie, David Gela, Marek Rodina, Roman Franěk, Martin Pšenička, Taiju Saito. Blastomeres derived from the vegetal pole provide extra-embryonic nutrition to sturgeon (Acipenser) embryos: Transition from holoblastic to meroblastic cleavage. Aquaculture (2022). 551, 737899. https://doi.org/10.1016/j.aquaculture.2022.737899.

Valihrach L, Matusova Z, Zucha D, Klassen R, Benesova S, Abaffy P, Kubista M and Anderova M (2022) Recent advances in deciphering oligodendrocyte heterogeneity with single-cell transcriptomics. Front. Cell. Neurosci. 16:1025012. doi: 10.3389/fncel.2022.1025012

Iegorova V, Naraine R, Psenicka M, Zelazowska M and Sindelka R (2022) Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis. Front. Cell Dev. Biol. 10:982732. doi: 10.3389/fcell.2022.982732

ISL1 is necessary for auditory neuron development and contributes toward tonotopic organization. Filova I, Pysanenko K, Tavakoli M, Vochyanova S, Dvorakova M, Bohuslavova R, Smolik O, Fabriciova V, Hrabalova P, Benesova S, Valihrach L, Cerny J, Yamoah EN, Syka J, Fritzsch B, Pavlinkova G. Proc Natl Acad Sci U S A. 2022 Sep 13;119(37):e2207433119. doi: 10.1073/pnas.2207433119.

Naraine R, Iegorova V, Abaffy P, Franek R, Soukup V, Psenicka M, Sindelka R. Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-Seq. Dev Biol. 2022 Jun 22:S0012-1606(22)00130-0. doi: 10.1016/j.ydbio.2022.06.013.

Gazo I, Naraine R, Lebeda I, Tomčala A, Dietrich M, Franěk R, Pšenička M, Šindelka R. Transcriptome and Proteome Analyses Reveal Stage-Specific DNA Damage Response in Embryos of Sturgeon (Acipenser ruthenus). International Journal of Molecular Sciences. 2022; 23(12):6392. https://doi.org/10.3390/ijms23126392

Smieszek A, Marcinkowska K, Pielok A, Sikora M, Valihrach L, Carnevale E, Marycz K. Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism. Cells. 2022; 11(9):1437. https://doi.org/10.3390/cells11091437

Small RNA-Sequencing for Analysis of Circulating miRNAs: Benchmark Study. Androvic P, Benesova S, Rohlova E, Kubista M, Valihrach L. J Mol Diagn. 2022 Jan 23:S1525-1578(22)00011-3. doi: 10.1016/j.jmoldx.2021.12.006.

Mitochondrial respiration supports autophagy to provide stress resistance during quiescence. Magalhaes-Novais S, Blecha J, Naraine R, Mikesova J, Abaffy P, Pecinova A, Milosevic M, Bohuslavova R, Prochazka J, Khan S, Novotna E, Sindelka R, Machan R, Dewerchin M, Vlcak E, Kalucka J, Stemberkova Hubackova S, Benda A, Goveia J, Mracek T, Barinka C, Carmeliet P, Neuzil J, Rohlenova K, Rohlena J. Autophagy. 2022 Mar 8:1-18. doi: 10.1080/15548627.2022.2038898.

Tureckova J, Kamenicka M, Kolenicova D, Filipi T, Hermanova Z, Kriska J, Meszarosova L, Pukajova B, Valihrach L, Androvic P, Zucha D, Chmelova M, Vargova L and Anderova M (2022) Compromised Astrocyte Swelling/Volume Regulation in the Hippocampus of the Triple Transgenic Mouse Model of Alzheimer’s Disease. Front. Aging Neurosci. 13:783120. doi: 10.3389/fnagi.2021.783120.

Isolation and Characterization of Highly Pure Type A Spermatogonia From Sterlet (Acipenser ruthenus) Using Flow-Cytometric Cell Sorting. Xie X, Tichopád T, Kislik G, Langerová L, Abaffy P, Šindelka R, Franěk R, Fučíková M, Steinbach C, Shah MA, Šauman I, Chen F, Pšenička M. Front Cell Dev Biol. 2021 Dec 10;9:772625. doi: 10.3389/fcell.2021.772625.

Zucha D, Kubista M, Valihrach L. Tutorial: Guidelines for Single-Cell RT-qPCR. Cells. 2021; 10(10):2607. https://doi.org/10.3390/cells10102607

Transient astrocyte-like NG2 glia subpopulation emerges solely following permanent brain ischemia. Kirdajova D, Valihrach L, Valny M, Kriska J, Krocianova D, Benesova S, Abaffy P, Zucha D, Klassen R, Kolenicova D, Honsa P, Kubista M, Anderova M. Glia. 2021 Jul 27. doi: 10.1002/glia.24064.

Benesova, S.; Kubista, M.; Valihrach, L. Small RNA-Sequencing: Approaches and Considerations for miRNA Analysis. Diagnostics 2021, 11, 964. https://doi.org/10.3390/diagnostics11060964.

Ultrasensitive circulating tumor DNA analysis enables precision medicine: experimental workflow considerations. Andersson D, Kristiansson H, Kubista M, Ståhlberg A. Expert Rev Mol Diagn. 2021 Mar 8:1-12. doi: 10.1080/14737159.2021.1889371.

Restriction of an intron size en route to endothermy. Královičová J, Borovská I, Pengelly R, Lee E, Abaffy P, Šindelka R, Grutzner F, Vořechovský I. Nucleic Acids Res. 2021 Feb 8:gkab046. doi: 10.1093/nar/gkab046.

Shah, M.A., Saito, T., Šindelka, R. et al. Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo. Fish Sci (2021).

Ancient Sturgeons Possess Effective DNA Repair Mechanisms: Influence of Model Genotoxicants on Embryo Development of Sterlet, Acipenser ruthenus. Gazo I, Franěk R, Šindelka R, Lebeda I, Shivaramu S, Pšenička M, Steinbach C. Int J Mol Sci. 2020 Dec 22;22(1):E6. doi: 10.3390/ijms22010006.

Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2. Huggett JF, Benes V, Bustin SA, Garson JA, Harris K, Kammel M, Kubista M, McHugh TD, Moran-Gilad J, Nolan T, Pfaffl MW, Salit M, Shipley G, Vallone PM, Vandesompele J, Wittwer C, Zeichhardt H. Clin Chem. 2020 Sep 7:hvaa214. doi: 10.1093/clinchem/hvaa214.

The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020. dMIQE Group. Clin Chem. 2020 Aug 1;66(8):1012-1029. doi: 10.1093/clinchem/hvaa125.