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Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2

Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2. Huggett JF, Benes V, Bustin SA, Garson JA, Harris K, Kammel M, Kubista M, McHugh TD, Moran-Gilad J, Nolan T, Pfaffl MW, Salit M, Shipley G, Vallone PM, Vandesompele J, Wittwer C, Zeichhardt H. Clin Chem. 2020 Sep 7:hvaa214. doi: 10.1093/clinchem/hvaa214.



The Digital MIQE Guidelines Update

The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020. dMIQE Group. Clin Chem. 2020 Aug 1;66(8):1012-1029. doi: 10.1093/clinchem/hvaa125.



Decoding the Transcriptional Response to Ischemic Stroke in Young and Aged Mouse Brain

Decoding the Transcriptional Response to Ischemic Stroke in Young and Aged Mouse Brain. Androvic P, Kirdajova D, Tureckova J, Zucha D, Rohlova E, Abaffy P, Kriska J, Valny M, Anderova M, Kubista M, Valihrach L. Cell Rep. 2020 Jun 16;31(11):107777. doi: 10.1016/j.celrep.2020.107777.



NormQ: RNASeq normalization based on RT-qPCR derived size factors

NormQ: RNASeq normalization based on RT-qPCR derived size factors. Naraine R, Abaffy P, Sidova M, Tomankova S, Pocherniaieva K, Smolik O, Kubista M, Psenicka M, Sindelka R. Comput Struct Biotechnol J. 2020 May 14;18:1173-1181. doi: 10.1016/j.csbj.2020.05.010. eCollection 2020.



Pre-analytical factors affecting the establishment of a single tube assay...

Pre-analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients. Schneegans S, Lück L, Besler K, Bluhm L, Stadler JC, Staub J, Greinert R, Volkmer B, Kubista M, Gebhardt C, Sartori A, Irwin D, Serkkola E, Af Hällström T, Lianidou E, Sprenger-Haussels M, Hussong M, Mohr P, Schneider SW, Shaffer J, Pantel K, Wikman H. Mol Oncol. 2020 May;14(5):1001-1015. doi: 10.1002/1878-0261.12669.



Liquid biopsy analysis in cancer diagnostics

Liquid biopsy analysis in cancer diagnostics. Andersson D, Kubista M, Ståhlberg A. Mol Aspects Med. 2020 Apr;72:100839. doi: 10.1016/j.mam.2019.100839.



Multicenter Evaluation of Circulating Cell-Free DNA Extraction and Downstream Analyses...

Multicenter Evaluation of Circulating Cell-Free DNA Extraction and Downstream Analyses for the Development of Standardized (Pre)analytical Work Flows. Lampignano R, Neumann MHD, ..., Kubista M, Ammerlaan W, Sprenger-Haussels M, Schlange T, Heitzer E; Innovative Medicines Initiative CANCER-ID Consortium. Clin Chem. 2019 Oct 18. pii: clinchem.2019.306837. doi: 10.1373/clinchem.2019.306837.



Performance Comparison of Reverse Transcriptases for Single-Cell Studies

Performance Comparison of Reverse Transcriptases for Single-Cell Studies. Daniel Zucha, Peter Androvic, Mikael Kubista, Lukas Valihrach.Clin Chem. 2020 Jan 1;66(1):217-228. doi: 10.1373/clinchem.2019.307835.



The role of nitric oxide during embryonic wound healing.

The role of nitric oxide during embryonic wound healing. Pavel Abaffy, Silvie Tomankova, Ravindra Naraine, Mikael Kubista & Radek Sindelka. BMC Genomics volume 20, Article number: 815 (2019).



High potassium exposure reveals the altered ability of astrocytes to regulate...

High potassium exposure reveals the altered ability of astrocytes to regulate their volume in the aged hippocampus of GFAP/EGFP mice. D. Kolenicova, J. Tureckova, B. Pukajova, L. Harantova, J. Kriska, D. Kirdajova, I. Vorisek, M. Kamenicka, L. Valihrach, P. Androvic, M. Kubista, L. Vargova, M. Anderova. Neurobiol Aging. 2020 Feb;86:162-181. doi: 10.1016/j.neurobiolaging.2019.10.009. Epub 2019 Oct 22.



Circulating miRNA analysis for cancer diagnostics and therapy.

Valihrach L, Androvic P, Kubista M. Circulating miRNA analysis for cancer diagnostics and therapy. Mol Aspects Med. 2020 Apr;72:100825. doi: 10.1016/j.mam.2019.10.002.



Multicentric Evaluation of Circulating Plasma MicroRNA Extraction Technologies...

Kloten V, Neumann MHD, Di Pasquale F, Sprenger-Haussels M, Shaffer JM, Schlumpberger M, Herdean A, Betsou F, Ammerlaan W, Af Hällström T, Serkkola E, Forsman T, Lianidou E, Sjöback R, Kubista M, Bender S, Lampignano R, Krahn T, Schlange T; CANCER-ID consortium. Clin Chem. 2019 Jun 24. pii: clinchem.2019.303271. doi: 10.1373/clinchem.2019.303271.



Detection of Abundant Non-Haematopoietic Circulating Cancer-Related Cells in Patients...

Kumar, J.; Chudasama, D.; Roberts, C.; Kubista, M.; Sjöback, R.; Chatterjee, J.; Anikin, V.; Karteris, E.; Hall, M. Detection of Abundant Non-Haematopoietic Circulating Cancer-Related Cells in Patients with Advanced Epithelial Ovarian Cancer. Cells 2019, 8, 732.



The legacy of sexual ancestors in phenotypic variability, gene expression and homoeolog...

The legacy of sexual ancestors in phenotypic variability, gene expression and homoeolog regulation of asexual hybrids and polyploids. Bartoš O, Röslein J, Kotusz J, Paces J, Pekárik L, Petrtýl M, Halačka K, Štefková Kašparová E, Mendel J, Boroń A, Juchno D, Leska A, Jablonska O, Benes V, Šídová M, Janko K. Mol Biol Evol. 2019 May 11. pii: msz114. doi: 10.1093/molbev/msz114.



Two-tailed RT-qPCR panel for quality control of circulating microRNA studies.

Androvic P, Romanyuk N, Urdzikova-Machova L, Rohlova E, Kubista M, Valihrach L. Two-tailed RT-qPCR panel for quality control of circulating microRNA studies. Sci Rep. 2019 Mar 12;9(1):4255. doi: 10.1038/s41598-019-40513-w.



Metformin Increases Proliferative Activity and Viability of Multipotent Stromal Stem...

Metformin Increases Proliferative Activity and Viability of Multipotent Stromal Stem Cells Isolated from Adipose Tissue Derived from Horses with Equine Metabolic Syndrome. Smieszek A, Kornicka K, Szłapka-Kosarzewska J, Androvic P, Valihrach L, Langerova L, Rohlova E, Kubista M, Marycz K. Cells. 2019; 8(2):80.



Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores...

Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells. Bajzikova M, Kovarova J, Coelho AR, Boukalova S, Oh S, Rohlenova K, Svec D, Hubackova S ... Kolar F, Kubista M, Truksa J, Dvorakova-Hortova K, Pacak K, Gurlich R, Stocker R, Zhou Y, Berridge MV, Park S, Dong L, Rohlena J, Neuzil J. Cell Metab. 2018 Nov 9. pii: S1550-4131(18)30646-6. doi: 10.1016/j.cmet.2018.10.014.



The contribution of TRPV4 channels to astrocyte volume regulation and brain edema...

The contribution of TRPV4 channels to astrocyte volume regulation and brain edema formation. Pivonkova H, Hermanova Z, Kirdajova D, Awadova T, Malinsky J, Valihrach L, Zucha D, Kubista M, Galisova A, Jirak D, Anderova M. Neuroscience. 2018 Oct 24. pii: S0306-4522(18)30691-2. doi: 10.1016/j.neuroscience.2018.10.028.



Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons.

Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons. Andersson D, Svec D, Pedersen C, Henriksen JR, Ståhlberg A. Int J Mol Sci. 2018 Oct 16;19(10). pii: E3185. doi: 10.3390/ijms19103185.



Circulating tumor cells mirror bone metastatic phenotype in prostate cancer.

Josefsson A, Larsson K, Månsson M, Björkman J, Rohlova E, Åhs D, Brisby H, Damber JE, Welén K. Oncotarget. Circulating tumor cells mirror bone metastatic phenotype in prostate cancer. 2018 Jun 29;9(50):29403-29413. doi: 10.18632/oncotarget.25634. eCollection 2018 Jun 29.



Adverse effects of Hif1a mutation and maternal diabetes on the offspring heart

Cerychova R, Bohuslavova R, Papousek F, Sedmera D, Abaffy P, Benes V, Kolar F, Pavlinkova G. Adverse effects of Hif1a mutation and maternal diabetes on the offspring heart. Cardiovasc Diabetol. 2018 May 12;17(1):68. doi: 10.1186/s12933-018-0713-0.



Alternative assembly of respiratory complex II connects energy stress to metabolic...

Bezawork-Geleta A, Wen H, Dong L, Yan B, Vider J, Boukalova S, Krobova L, Vanova K, Zobalova R, Sobol M, Hozak P, Novais SM, Caisova V, Abaffy P, Naraine R, Pang Y, Zaw T, Zhang P, Sindelka R, Kubista M, Zuryn S, Molloy MP, Berridge MV, Pacak K, Rohlena J, Park S, Neuzil J. Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints. Nat Commun. 2018 Jun 7;9(1):2221. doi: 10.1038/s41467-018-04603-z.



Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg ...

Sindelka, R., Abaffy, P., Qu, Y., Tomankova, S., Sidova, M., Naraine, R., Kolar, M., Peuchen, E., Sun, L., Dovichi, N., Kubista, M. Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg and their impact on the developmental plan. Scientific Reports, 8: 8315, 2018. doi: 10.1038/s41598-018-26592-1. ISSN: 2045-2322.



Gene Expression Analysis of Immunomagnetically Enriched Circulating Tumor Cell Fraction...

Skerenova, M., Mikulova, V., Capoun, O., Svec, D., Kolostova, K., Soukup, V., Honova, H.,Hanus, T., Zima, T. Gene Expression Analysis of Immunomagnetically Enriched Circulating Tumor Cell Fraction in Castration-Resistant Prostate Cancer. Molecular Diagnosis & Therapy, 22 (3): 381–390, 2018 . doi: 10.1007/s40291-018-0333-0. ISSN: 1177-1062



Identification of inhibitors regulating cell proliferation and FUS-DDIT3 expression ...

Svec, D., Dolatabadi, S., Thomsen, C., Cordes, N., Shannon, M., Fitzpatrick, P., Landberg, G., Aman, P., Stahlberg, A. Identification of inhibitors regulating cell proliferation and FUS-DDIT3 expression in myxoid liposarcoma using combined DNA, mRNA, and protein analyses. Laboratory Investigation 2018 Mar 27. doi: 10.1038/s41374-018-0046-3.



The secrets of the cell

Kubista, M., Dreyer-Lamm, J., Stahlberg, A. The secrets of the cell. Molecular Aspects of Medicine, 59: 1-4, 2018. doi: 10.1016/j.mam.2017.08.004. ISSN: 0098-2997.



Comparison of oocyte mRNA localization patterns in sterlet Acipenser ruthenus and ...

Pochnerniaieva, K., Sidova, M., Havelka, M., Saito, M., Psenicka, M., Sindelka, R., Kaspar, V. Comparison of oocyte mRNA localization patterns in sterlet Acipenser ruthenus and African clawed frog Xenopus laevis. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, [ahead of print], 2018. doi: 10.1002/jez.b.22802. ISSN 1552-5015.



Technical aspects and recommendations for single-cell qPCR

Stahlberg, A., Kubista, M. Technical aspects and recommendations for single-cell qPCR. Molecular Aspects of Medicine, 59: 28-35, 2018. doi: 10.1016/j.mam.2017.07.004. ISSN: 0098-2997.



Platforms for Single-Cell Collection and Analysis

Valihrach, L., Androvic, P., Kubista, M. Platforms for Single-Cell Collection and Analysis. International Journal of Molecular Sciences, 19(3): 807, 2018. doi: 10.3390/ijms19030807. ISSN 1422-0067.



A single-cell analysis reveals multiple roles of oligodendroglial lineage cells during ...

Valny, M., Honsa, P., Waloschkova, E., Matuskova, H., Kriska, J., Kirdajova, D., Androvic, P., Valihrach, L., Kubista, M., Anderova, M. A single-cell analysis reveals multiple roles of oligodendroglial lineage cells during post-ischemic regeneration. Glia, 66(5): 1068-1081, 2018. doi: 10.1002/glia.23301. ISSN 1098-1136.