Pavel ABAFFY, PhD
I am a Ph.D. student in the group of Radek Sindelka. I study the process of embryonic wound healing and regeneration in the embryos of the frog model Xenopus laevis. I focus mainly on the method for the measurement of gene expression, especially using RNA-Seq. I assist people throughout each step of the process of this method – experimental design, sample preparation, library preparation, sequencing and data analysis. I like optimization of processes, especially those involving challenging samples (isolation in specific condition or from complicated tissue, usage of kits out of their declared limits) or “exotic” samples. Due to my interest in Informatics and Mathematics, I started to learn and explore many aspects about NGS (especially RNA-Seq) and now I assist people with their questions about this popular method. My big advantage is that I am well versed in all aspects of the RNA-Seq process, from the experimental design part, continuing with isolation and library preparation and finishing with verification of results, so I can help people with almost any crazy question they can have for their experiment.