MSc. Šárka Benešová

Biography

I am a M.Sc. student of Bioinformatics and Cheminformatics at UCT (University of Chemistry and Technology). I am also part of the Laboratory of Gene Expression, where I work with Lukáš Valihrach on the processing and statistical analysis of data from single cell RNA sequencing. As this method has become quite important in cell characterization during the last few years, my thesis focuses on the creation of a generic pipeline that can be easily used for the processing of our data. I already have another Master's degree from the Charles University which focused on experimental plant biology. Therefore I have plenty of experiences with wet lab techniques like immunohistochemical detection of proteins, RNA extraction, Northern blotting, cloning, RT-qPCR, in situ hybridization etc. However, I prefer to process data more than to generate them 😊 so I decided to educate myself more at UCT, and as such have led to to where I am today.

Since the work of a bioinformatician is basically sitting on a chair in front of a computer, I like to move in my spare time which includes mainly hiking, climbing and pole dancing.

Publications

1. Small RNA-Sequencing for Analysis of Circulating miRNAs: Benchmark Study. Androvic P, Benesova S, Rohlova E, Kubista M, Valihrach L. J Mol Diagn. 2022 Jan 23:S1525-1578(22)00011-3. doi: 10.1016/j.jmoldx.2021.12.006.
2. Transient astrocyte-like NG2 glia subpopulation emerges solely following permanent brain ischemia. Kirdajova D, Valihrach L, Valny M, Kriska J, Krocianova D, Benesova S, Abaffy P, Zucha D, Klassen R, Kolenicova D, Honsa P, Kubista M, Anderova M. Glia. 2021 Jul 27. doi: 10.1002/glia.24064.
3. An Integrative Study of Aortic mRNA/miRNA Longitudinal Changes in Long-Term LVAD Support. Dlouha D, Ivak P, Netuka I, Benesova S, Tucanova Z, Hubacek JA. Int J Mol Sci. 2021 Jul 10;22(14):7414. doi: 10.3390/ijms22147414.
4. Small RNA-Sequencing: Approaches and Considerations for miRNA Analysis. Benesova S, Kubista M, Valihrach L. Diagnostics (Basel). 2021 May 27;11(6):964. doi: 10.3390/diagnostics11060964.