Small RNA-Sequencing for Analysis of Circulating miRNAs: Benchmark Study. Androvic P, Benesova S, Rohlova E, Kubista M, Valihrach L. J Mol Diagn. 2022 Jan 23:S1525-1578(22)00011-3. doi: 10.1016/j.jmoldx.2021.12.006.
Small RNA-sequencing (RNA-Seq) is being increasingly used for profiling of circulating microRNAs (miRNAs), a new group of promising biomarkers. Unfortunately, small RNA-Seq protocols are prone to biases limiting quantification accuracy, which motivated development of several novel methods. Here, the authors present comparison of all small RNA-Seq library preparation approaches that are commercially available for quantification of miRNAs in biofluids. Using synthetic and human plasma samples, performance of traditional two-adaptor ligation protocols (Lexogen, Norgen), as well as methods using randomized adaptors (NEXTflex), polyadenylation (SMARTer), circularization (RealSeq), capture probes (EdgeSeq), or unique molecular identifiers (QIAseq), were compared. There was no single protocol outperforming others across all metrics. The authors documented limited overlap of measured miRNA profiles between methods largely owing to protocol-specific biases. It was found that methods designed to minimize bias largely differ in their performance, and contributing factors were identified. It was found that usage of unique molecular identifiers has rather negligible effect and, if designed incorrectly, can even introduce spurious results. This study's results identify strengths and weaknesses of all current methods and provide guidelines for applications of small RNA-Seq in biomarker research.
Laboratory of Gene Expression
Institute of Biotechnology CAS, v.v.i.
252 50 Vestec
Phone: +420 325 873 748, +420 325 873 747