Breadcrumb navigation

Home > News > Two-tailed PCR licensed by BioVendor

Two-tailed PCR licensed by BioVendor

We are happy to announce that the Two-tailed technology for microRNA analysis developed in the Laboratory of Gene Expression1,2 in collaboration with TATAA Biocenter has been recently licensed by Czech biotechnology company BioVendor. BioVendor is a provider of a comprehensive solution for miRNA-oriented researchers. In addition to research, BioVendor is enabling the miRNA biomarkers to enter clinical diagnostic applications. The highly specific and sensitive Two-Tailed PCR technology provides BioVendor with rock-solid base to establish own diagnostic miRNA panels, broaden the life science portfolio, and kick-start BioVendor's rapid expansion in the miRNA market. For more info, see


Schematic of Two-tailed RT-qPCR. (A) Two-tailed RT primer having two hemiprobes connected by a hairpin folding sequence. (B) The hemiprobes bind cooperatively, one at each end of the target miRNA, forming a stable complex. (C) Reverse transcriptase binds the 3′-end of the hybridized Two-tailed RT primer and elongates it to form tailed cDNA. (D) The cDNA is amplified by qPCR using two target-specific primers.


1. Androvic, P., Valihrach, L., Elling, J., Sjoback, R., Kubista, M., 2017. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Research 45 (15).

2. Androvic, P., Romanyuk, N., Urdzikova-Machova, L., Rohlova, E., Kubista, M., Valihrach, L., 2019. Two-tailed RT-qPCR panel for quality control of circulating microRNA studies. Sci Rep-Uk 9.