Our laboratory published new study: Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-Seq
Naraine R, Iegorova V, Abaffy P, Franek R, Soukup V, Psenicka M, Sindelka R. Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-Seq. Dev Biol. 2022 Jun 22:S0012-1606(22)00130-0. doi: 10.1016/j.ydbio.2022.06.013. Epub ahead of print. PMID: 35752299.
Asymmetrical localization of biomolecules inside the egg, results in uneven cell division and establishment of many biological processes, cell types and the body plan. However, our knowledge about evolutionary conservation of localized transcripts is still limited to a few models. Our goal was to compare localization profiles along the animal-vegetal axis of mature eggs from four vertebrate models, two amphibians (Xenopus laevis, Ambystoma mexicanum) and two fishes (Acipenser ruthenus, Danio rerio) using the spatial expression method called TOMO-Seq. We revealed that RNAs of many known important transcripts such as germ layer determinants, germ plasm factors and members of key signalling pathways, are localized in completely different profiles among the models. It was also observed that there was a poor correlation between the vegetally localized transcripts but a relatively good correlation between the animally localized transcripts. These findings indicate that the regulation of embryonic development within the animal kingdom is highly diverse and cannot be deduced based on a single model.
Laboratory of Gene Expression
Institute of Biotechnology CAS, v.v.i.
252 50 Vestec
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