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David Švec, Ph.D.
Postdoc / Scientist
In the Laboratory of Gene Expression, Institute of Biotechnology, AV CR Prague, I am focused on single cells analysis and creating a map of mRNA distribution in time and space using qPCR tomography in developing mouse tooth. I have utilized a combination of qPCR tomography with laser microdissection to reveal co-localization of the most important developmental genes (SHH, FGF, BMP and Pax families). I have also been involved in the production of an optimised protocol for dye based analysis in microfluidic systems and I have also developed panels of assays and tools for assessing the quality of RNA within paneuropean project SPIDIA. I am also responsible for organising qPCR courses. Currently I am involved in the research of transport of mitochondria in cancer on single cell level using automated single cell picking and digital PCR; cancer and healing; consulting and supporting Genecore facility customers.