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Evidence-based quality guidelines for pre-analytical phase of blood samples

Pazzagli, M., Malentacchi, F., Simi, L., Orlando, C., Wyrich, R., Gunther, K., Hartmann, C., Verderio, P., Pizzamiglio, S., Ciniselli, C. M., Tichopad, A., Kubista, M., Gelmini, S. Evidence-based quality guidelines for pre-analytical phase of blood samples. Clinical Chemistry and Laboratory Medicine, 49(1): 128, 2011. ISSN 1434-6621.



Preconceptional paternal glycidamide exposure affects embryonic gene expression: ...

Brevik, A., Rusnakova, V., Duale, N., Slagsvold, H. H., Olsen, A.-K., Storeng, R., Kubista, M., Brunborg, G., Lindeman, B. Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization. Reproductive Toxicology, 32: 463-471, 2011. ISSN 0890-6238.



Primer Sequence Disclosure: A Clarification of the MIQE Guideline

Bustin, A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl , M. W., Shipley, G. L., Vandesompele, J., Wittwer, C. T. Primer Sequence Disclosure: A Clarification of the MIQE Guideline. Clinical Chemistry, 57: 919-921, 2011. ISSN 0009-9147.



Single-cell gene-expression profiling and its potential diagnostic application

Stahlberg, A., Kubista, M., Aman, P. Single-cell gene-expression profiling and its potential diagnostic application. Expert Review of Molecular Diagnostics, 11: 735-740, 2011. ISSN 1473-7159.



DNA diagnostics get digitased

Kubista, M., Stahlberg, A. DNA diagnostics get digitased. Drug Discovery World, 2011.



qPCR Round Table

Kubista, M., Tichopad, A. qPCR Round Table. International Drug Discovery 2011.



Fate of Cry1Ab Protein in Agricultural Systems under Slurry Management of Cows Fed ...

Gruber, H., Paul, V., Guertler, P., Spiekers, H., Tichopad, A., Meyer, H. H. D., Muller M. Fate of Cry1Ab Protein in Agricultural Systems under Slurry Management of Cows Fed Genetically Modified Maize (Zea mays L.) MON810: A Quantitative Assessment. J Agric Food Chem 2011.



High throughput single cell expression profiling: Taking a closer look on biological ...

Kubista, M., Rusnakova, V., Svec, D., Stahlberg, A. High throughput single cell expression profiling: Taking a closer look on biological response. Clinical Chemistry and Laboratory Medicine, 49(1): 118, 2011. ISSN 1434-6621.



High throughput single cell expression profiling: Taking a closer look on biological ...

Kubista, M., Strombom, L., Svec, D., Rusnakova, V., Stahlberg, A. High throughput single cell expression profiling: Taking a closer look on biological response. European Pharmaceutical Review, 16(2): 20-24, 2011.



The role of disseminated tumor cells and circulating tumor cells in management of ...

Mikulova, V., Cabinakova, M., Pobudova, J., Tesarova, P., Pavlista, D., Kolostova, K., Kubista, M., Romzova, M., Zima, T. The role of disseminated tumor cells and circulating tumor cells in management of breast cancer patients. Tumor Biology, 31(1): 73, 2010. ISSN 1010-428.



Gene expression profiling – Clusters of possibilities

Bergkvist, A., Rusnakova, V., Sindelka, R., Garda, J. M. A., Sjogreen, B., Lindh, D., Forootan, A., Kubista, M. Gene expression profiling – Clusters of possibilities. Methods, 50: 323-335, 2010. ISSN 1046-2023.



Gene expression profiling of sex differences in HIF1-dependent adaptive cardiac ...

Bohuslavova, R., Kolar, F., Kuthanova, L., Neckar, J., Tichopad, A., Pavlinkova, G. Gene expression profiling of sex differences in HIF1-dependent adaptive cardiac responses to chronic hypoxia. Journal of Applied Physiology, 109: 1195-1202, 2010. ISSN 8750-7587.



Natural variation explains most transcriptomic changes among maize plants of MON810 and...

Coll, A., Nadal, A., Collado, R., Capellades, G., Kubista, M., Messeguer, J., Pla, M. Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practice. Plant Molecular Biology, 73: 349-362, 2010. ISSN 0167-4412.



Statistical aspects of quantitative real-time PCR experiment design

Kitchen, R. R., Kubista, M., Tichopad, A. Statistical aspects of quantitative real-time PCR experiment design. Methods, 50: 231-236, 2010. ISSN 1046-2023.



Analysis of In Vitro and In Vivo Characteristics of Human Embryonic Stem Cell-Derived ...

Kozubenko, N., Turnovcova, K., Kapcalova, M., Butenko, O., Anderova, M., Rusnakova, V., Kubista, M., Hampl, A., Jendelova, P., Sykova, E. Analysis of In Vitro and In Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural Precursor. Cell Transplantation, 19: 471-486, 2010. ISSN 0963-6897.



Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography

Sindelka, R., Sidova, M., Svec, D., Kubista, M. Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography. Methods, 51: 87-91, 2010. ISSN 1046-2023.



Quality control for quantitative PCR based on amplification compatibility test

Tichopad, A., Bar, T., Pecen, L., Kitchen, R. R., Kubista, M., Pfaffl , M. W. Quality control for quantitative PCR based on amplification compatibility test. Methods, 50: 308-312, 2010. ISSN 1046-2023.



Adjuvant effect of Bacillus firmus on the expression of cytokines and toll-like ...

Zanvit, P., Tichopad, A., Havlickova, M., Novotna, O., Jirkovska, M., Kolostova, K., Cechova, D., Julak, J., Sterzl, I., Prokesova, L.Adjuvant effect of Bacillus firmus on the expression of cytokines and toll-like receptors in mouse nasopharynx-associated lymphoid tissue (NALT) after intranasal immunization with inactivated influenza virus type A. Immunology Letters, 134: 26-34, 2010. ISSN 0165-2478.



Antimalarial Exposure Delays Plasmodium falciparum Intra-Erythrocytic Cycle and ...

Veiga, M. I., Ferreira, P. E., Schmidt, B. A., Ribacke, U., Bjorkman, A., Tichopad, A., Gil, J. P. Antimalarial Exposure Delays Plasmodium falciparum Intra-Erythrocytic Cycle and Drives Drug Transporter Genes Expression. PLoS ONE, 5(8): e12408, 2010. ISSN 1932-6203.



Defining cell populations with single-cell gene expression profiling: correlations and ...

Stahlberg, A., Andersson, D., Aurelius, J., Faiz, M., Pekna, M., Kubista, M., Pekny, M., Defining cell populations with single-cell gene expression profiling: correlations and identification of astrocyte subpopulations. Nucleic Acids Research, 39(4): 1-12, 2010. ISSN 0305-1048.



Gene expression profiling in circulation cells (CTCS) of breast carcinoma patients

Kolostova, K., Pinterova, D., Tesarova, P., Bobek, V., Mikulova, V., Kubecova, M., Brychta, M., Rusnakova, V., Bauer, S. K., Kubista, M. Gene expression profiling in circulation cells (CTCS) of breast carcinoma patients. Annals of Oncology, 21(4): 49-50, 2010.



Effect of feeding status on adjuvant arthritis severity, cachexia, and insulin ...

Stofkova, A., Zelezna, B., Romzova, M., Ulicna, O., Kiss, A., Skurlova, M., Jurcovicova, J. Effect of feeding status on adjuvant arthritis severity, cachexia, and insulin sensitivity in male Lewis rats. Mediators of Inflammation, Article No.: 398026, 2010. ISSN 0962-9351.



Real-time PCR: Where we are and where are we heading

Kubista, M. Real-time PCR: Where we are and where are we heading. Drug Discovery World Spring 2010.



Efficacy of S-adenosylhomocysteine hydrolase inhibitors, D-eritadenine and (S)-DHPA, ...

Ctrnacta, V., Fritzler, J. M., Surinova, M., Hrdy, I., Zhu, G., Stejskal, F. Efficacy of S-adenosylhomocysteine hydrolase inhibitors, D-eritadenine and (S)-DHPA, against the growth of Cryptosporidium parvum in vitro. Experimental Parasitology, 126(2): 113-116, 2010. ISSN:0014-4894.



The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR

Bustin, A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl , M. V., Shipley, G. L., Vandesompele, J., Wittver, C. T. The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR. Experiment Clinical Chemistry, 55: 611-622, 2009. ISSN 0009-9147.



Gene Expression Responses in Larvae of the Fleshfly Sarcophaga bullata after Immune ...

Masova, A., Sindelka, R., Kubista, M., Kindl, J., Jiracek, J. Gene Expression Responses in Larvae of the Fleshfly Sarcophaga bullata after Immune Stimulation. Folia Biologica, 55: 98-106, 2009. ISSN 0015-5500.



Design and Optimization of Reverse-Transcription Quantitative PCR

Tichopad, A., Kitchen, R., Riedmaier, I., Becker, Ch., Ståhlberg, A., Kubista, M. Design and Optimization of Reverse-Transcription Quantitative PCR. Experiment Clinical Chemistry, 55:1816-1823, 2009. ISSN 0009-9147.



Data Analysis Software: Chapter 5 in Real-Time PCR: Current Technology and Applications

Pfaffl, M. V., Vandesompele, J., Kubista, M. Data Analysis Software: Chapter 5 in Real-Time PCR: Current Technology and Applications. Caister Academic Press, editor: Logan, J., Edwards, K., Saunders, N., 2009. ISBN 978-1-904455-39-4.



Intracellular Gene Expression Profiles Revealed with Real-time PCR Tomography

Kubista, M. Intracellular Gene Expression Profiles Revealed with Real-time PCR Tomography. Research Spotlight:Gene Expression, 2009.



What future holds for real-time PCR

Kubista, M. What future holds for real-time PCR. European Pharmaceutical Reviews Industry Focus, 2009.