Publications

Kubista, M. Prime time for qPCR – raising the quality bar. European Pharmaceutical Review, 19(3): 63-67, 2014. 

Kubista, M., Sjoback, R., Jindrichova, M., Rohlova, E., Rusnakova, V., Hauch, S., Aktas, B., Tewes, M., Bredemeier, M., Kasimir-Bauer, S. High Throughput Few and Single Cell Expression Profiling and the Characterization of Circulating Tumor Cells. Anticancer Research, 34(10): 6014, 2014. Abstracts of the Ninth International Conference of Anticancer Research, October 6-10, 2014, Sithonia, Greece, Meeting Abstract: 350. ISSN 0250-7005.

Honsa, P., Pivonkova, H., Harantova, L., Butenko, O., Kriska, J., Dzamba, D., Rusnakova, V., Valihrach, L., Kubista, M., Anderova, M. Increased Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in Reactive Astrocytes FollowingIschemia. GLIA, 62(12): 2004-2021, 2014. doi: 10.1002/glia.22721. ISSN 1098-1136.

Conrad, S., Azizi, H., Hatami, M., Kubista, M., Bonin, M., Hennenlotter, J., Renninger, M., Skutella, T. Differential gene expression profiling of enriched human spermatogonia after short- and long-term culture. Biomed Research International, 2014. Article Number: 138350, doi: 10.1155/2014/138350. ISSN 2314-6133. eISSN 2314-6141.

Slyskova, J., Cordero, F., Pardini, B., Korenkova, V., Vymetalkova ,V., Bielik, L., Vodickova, L., Pitule, P., Liska, V., Matejka, V. M., Levy, M., Buchler, T., Kubista, M., Naccarati, A., Vodicka, P. Post-treatment recovery of suboptimal DNA repair capacity and gene expression levels in colorectal cancer patients. Molecular Carcinogenesis, 2014.

Vymetalkova, V. P., Slyskova, J., Korenkova ,V., Bielik, L., Langerova, L., Prochazka, P., Rejhova, A., Schwarzova, L., Pardini, B., Naccarati, A., Vodicka, P. Molecular characteristics of mismatch repair genes in sporadic colorectal tumors in Czech patients. BMC Med Genet., 15(1):17, 2014. doi: 10.1186/1471-2350-15-17. ISSN 1471-2350.

Zatecka, E., Ded, L., Elzeinova, F., Kubatova, A., Dorosh, A., Margaryan, H., Dostalova, P., Korenkova, V., Hoskova, K., Peknicova, J. Effect of zearalenone on reproductive parameters and expression of selected testicular genes in mice. Reproductive toxicology, 45: 20-30, 2014. doi:10.1016/j.reprotox.2014.01.003. ISSN 1873-1708.

Stahlberg, A., Kubista, M. The workflow of single-cell expression profiling using quantitative real-time PCR. Expert Review of Molecular Diagnostics, 14(3): 323–331, 2014. doi: 10.1586/14737159.2014.901154. ISSN 1473-7159.

Stahlberg, A., Aman, P., Strombom, L., Zoric, N., Diez, A., Nilsson, O., Kubista, M., Ridell, B. Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas. Hindawi Publishing Corporation, 2014: 1-6, 2014.

Sorg, G., Danowski, K., Korenkova, V., Rusnakova, V., Kueffner, R., Zimmer, R., Meyer, H. H. D., Kliem, H. Microfluidic high-throughput RT-qPCR measurements of the immune response of primary bovine mammary epithelial cells cultured from milk to mastitis pathogens. Animal, 7(5): 799-805, 2013. ISSN 1751-7311.

Bustin, S. A., Benes, V., Garson, J., Hellemans, J., Huggett, J., Kubista,... The need for transparency and good practices in the qPCR literature. Nature Methods, 10: 1063–1067, 2013. ISSN 1548-7091.

Svec, D., Andersson, D., Pekny M., Sjoback, R., Kubista, M., Stahlberg, A. Direct cell lysis for single-cell gene expression profiling. Front. Oncol, 3(274): 1-11, 2013.

Svec, D., Rusnakova, V., Korenkova, V., Kubista, M. Dye-based High-Throughput qPCR in Microfluidic Platform BioMark. In PCR Technology, Nolan, T. (ed.). CRC Press Inc., 323-339, 2013. ISBN 9781439848050.

Butenko, O., Benesova, J., Mikesova, M., Honsa, P., Dzamba, D., Kriska, J., Rusnakova, V., Kubista, M., Anderova, M. The fate of mdach1-expressing cells in the dorsal part of the lateral ventricles following focal cerebral ischemia. GLIA, 61(1): 207, 2013. ISSN 0894-1491.

Huggett, J. F., Foy, C. A., Benes, V., Emslie, K., Garson, J. A., Haynes, R., Hellemans, J., Kubista, M., Mueller, R. D., Nolan, T., Pfaffl, M. W., Shipley, G. L., Vandesompele, J., Bustin, S. A. The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments. Clinical Chemistry, 59(6): 892-902, 2013. ISSN 0009-9147.

Stahlberg, A, Rusnakova, V, Kubista, M. The added value of single-cell gene expression profiling. Brief Funct Genomics, 12(2): 81-89, 2013. ISSN 2041-2649.

Stahlberg, A., Rusnakova, V., Forootan, A., Anderova, M., Kubista, M. RT-qPCR work-flow for single-cell data analysis. Methods, 59(1): 80-88, 2013, ISSN 1046-2023.

Pazzagli, M., Malentacchi, F., Simi, L., Wyrich, R., Guenther, K., Hartmann, C., Verderio, P., Pizzamiglio, S., Ciniselli, C. M., Tichopad, A., Kubista, M., Gelmini, S. SPIDIA-RNA: First external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses. Methods, 59(1): 20-31, 2013. ISSN 1046-2023.

Stahlberg, A., Kubista, M. Unrevealing biological secrets by single cell expression profilig. Drug Discovery World Summer, 2013.

Andersson, D., Wilhelmsson, U., Nilsson, M., Kubista, M., Stahlberg, A., Pekna, M., Pekny, M. Plasticity response in the contralesional hemisphere after subtle neurotrauma: gene expression profiling after partial deafferentation of the hippocampus. PLoS ONE, 8(7): e706992013, 2013. ISSN 1932-6203.

Flachsova, M., Sindelka, R., Kubista, M. Single blastomere expression profiling of Xenopus laevis embryos of 8 to 32-cells reveals developmental asymmetry. Scientific Reports, 3: 2278, 2013. ISSN: 2045-2322.

Virant-Klun, I., Skutella, T., Kubista, M., Vogler A., Sinkovec, J., Meden-Vrtovec, H. Expression of Pluripotency and Oocyte-Related Genes in Single Putative Stem Cells from Human Adult Ovarian Surface Epithelium Cultured In Vitro in the Presence of Follicular Fluid. BioMed Research International, 861460, 2013. ISSN: 2314-6133.

Rusnakova, V., Honsa, P., Dzamba, D., Stahlberg A., Kubista, M., Anderova, M. Heterogeneity of Astrocytes: From Development to Injury – Single Cell Gene Expression. PLoS ONE 8(8): e69734, 2013. ISSN 1932-6203.

Malentacchi, F., Pazzagli, M., Simi, L., Orlando, C., Wyrich, R., Hartmann, C. C., Verderio, P., Pizzamiglio, S., Ciniselli, C. M., Tichopad, A., Kubista, M., Gelmini, S. SPIDIA-DNA: An External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses. Clinica Chimica Acta 424: 274–286, 2013. ISSN 0009-8981.

Dusatkova, L., Zamrazilova, H., Sedlackova, B., Vcelak, J., Hlavaty, P., Aldhoon Hainerova, I., Korenkova, V., Bradnova, O., Bendlova, B., Kunesova, M., Hainer, V. Association of obesity susceptibility gene variants with metabolic syndrome and related traits in 1,443 czech adolescents. Folia Biologica 59: 123-133, 2013. ISSN 0015-5500.

Polakova Vymetalkova, V., Vannucci, L., Korenkova, V., Prochazka, P., Slyskova, J., Vodickova, L., Rusnakova, V., Bielik, L., Burocziova, M., Rossmann, P., Vodicka, P. Evaluation of tumor suppressor gene expressions and aberrant methylation in the colon of cancer-induced rats: a pilot study. Molecular Biology Reports, 40(10): 5921-5929, 2013. doi: 10.1007/s11033-013-2699-8. ISSN 0301-4851.

Bar, T., Kubista, M., Tichopad, A. Validation of kinetics similarity in qPCR. Nucleic Acids Research, 40: 1395-1406, 2012. ISSN 0305-1048. 

Benesova, J., Rusnakova, V., Honsa, P., Pivonkova, H., Dzamba, D., Kubista, M., Anderova, M. Distinct expression/function of potassium and chloride channels contributes to the diverse volume regulation in cortical astrocytes of GFAP/EGFP mice. PLoS ONE, 7: e29725, 2012. ISSN 1932-6203. 

Bibova, I., Linhartova, I., Stanek, O., Rusnakova, V., Kubista, M., Suchanek, M., Vasakova, M., Sebo, P. Detection of immune cell response to M. tuberculosis-specific antigens by quantitative polymerase chain reaction. Diagnostic Microbiology and Infectious Disease, 72: 68-78, 2012. ISSN 0732-8893. 

Guenther, K., Malentacchi, F., Verderio, P., Pizzamiglio, S., Ciniselli, Ch. M., Tichopad, A., Kubista, M., Wyrich, R., Pazzagli, M., Gelmini, S. Implementation of a proficiency testing for the assessment of the preanalytical phase of blood samples used for RNA based analysis. Clinica Chimica Acta, 413: 779-786, 2012. ISSN 0009-8981.