Publications

Korenkova, V., Scott, J., Novosadova, V., Jindrichova, M., Langerova, L., Svec, D., Sidova, M., Sjoback, R. Pre-amplification in the context of high-throughput qPCR gene expression experiment. BMC Molecular Biology, 16: 5, 2015. doi: 10.1186/s12867-015-0033-9. ISSN 1471-2199.

Kubista, M. Addressing biological heterogeneity with single cell profiling. European Pharmaceutical Review, 20(3): In-depth focus: PCR, 2015.

Sidova, M., Sindelka, R., Castoldi, M., Benes, V., Kubista, M. Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division. Scientific Reports, 5: 11157, 2015. doi: 10.1038/srep11157. eISSN 2045-2322.

Sidova, M., Tomankova, S., Abaffy, P., Kubista, M., Sindelka, R. Effects of post-mortem and physical degradation on RNA integrity and quality. Biomolecular Detection and Quantification, 5: 3-9, 2015. doi: 10.1016/j.bdq.2015.08.002. ISSN 2214-7535.

Svec, D., Tichopad, A., Novosadova, V., Pfaffl, M. W., Kubista, M. How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments. Biomolecular Detection and Qualification, 3: 9-16, 2015. doi:10.1016/j.bdq.2015.01.005. ISSN 2214-7535.

Kalle, E., Kubista, M., Rensing, Ch. Multi-template polymerase chain reaction. Biomolecular Detection and Qualification, 2: 11-29, 2014. doi:10.1016/j.bdq.2014.11.002. ISSN 2214-7535.

Anderova, M., Benesova, J., Mikesova, M., Dzamba, D., Honsa, P., Kriska, J., Butenko, O., Novosadova, V., Valihrach, L., Kubista, M., Dmytrenko, L., Cicanic, M., Vargova, L. Altered Astrocytic Swelling in the Cortex of α-Syntrophin-Negative GFAP/EGFP Mice. PLoS ONE, 9(11): e113444, 2014. doi: 10.1371/journal.pone.0113444. ISSN 1932-6203.

Slyskova, J., Lorenzo, Y., Karlsen, A., Carlsen, M. H., Novosadova, V., Blomhoff, R., Vodicka, P., Collins, A. R. Both genetic and dietary factors underlie individual differences in DNA damage levels and DNA repair capacity. DNA Repair, 16(2014): 66-73, 2014. doi: 10.1016/j.dnarep.2014.01.016. ISSN 1568-7864.

Malentacchi, F., Pazzagli, M., Simi, M., Orlando, C., Wyrich, R., Gunther, K., Verderio, P., Pizzamiglio, S., Ciniselli, Ch. M., Zhang, H., Korenkova, V., Rainen, L., Bar, T., Kubista, M., Gelmini, S. SPIDIA-RNA: Second External Quality Assessment for the Pre-Analytical Phase of Blood Samples Used for RNA Based Analyses. PLoS ONE 9(11): e112293. doi:10.1371/journal.pone.0112293. ISSN 1932-6203.

Zhang, H., Korenkova, V., Sjoback, R., Svec, D., Bjorkman, J., Kruhoffer, M., Verderio, P., Pizzamiglio, S., Ciniselli, Ch. M., Wyrich, R., Oelmueller, U., Kubista, M., Lindahl, T., Lonneborg, A., Rian, E. Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples. PLoS ONE 9(11): e111644, 2014. doi:10.1371/journal.pone.0111644. ISSN 1932-6203.

Jacox, L., Sindelka, R., Chen, J., Rothman, A., Dickinson, A., Sive, H. The Extreme Anterior Domain Is an Essential Craniofacial Organizer Acting through Kinin-Kallikrein Signaling. Cell Reports, 8(2): 596-609, 2014.

Kubista, M. Prime time for qPCR – raising the quality bar. European Pharmaceutical Review, 19(3): 63-67, 2014. 

Kubista, M., Sjoback, R., Jindrichova, M., Rohlova, E., Rusnakova, V., Hauch, S., Aktas, B., Tewes, M., Bredemeier, M., Kasimir-Bauer, S. High Throughput Few and Single Cell Expression Profiling and the Characterization of Circulating Tumor Cells. Anticancer Research, 34(10): 6014, 2014. Abstracts of the Ninth International Conference of Anticancer Research, October 6-10, 2014, Sithonia, Greece, Meeting Abstract: 350. ISSN 0250-7005.

Honsa, P., Pivonkova, H., Harantova, L., Butenko, O., Kriska, J., Dzamba, D., Rusnakova, V., Valihrach, L., Kubista, M., Anderova, M. Increased Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in Reactive Astrocytes FollowingIschemia. GLIA, 62(12): 2004-2021, 2014. doi: 10.1002/glia.22721. ISSN 1098-1136.

Conrad, S., Azizi, H., Hatami, M., Kubista, M., Bonin, M., Hennenlotter, J., Renninger, M., Skutella, T. Differential gene expression profiling of enriched human spermatogonia after short- and long-term culture. Biomed Research International, 2014. Article Number: 138350, doi: 10.1155/2014/138350. ISSN 2314-6133. eISSN 2314-6141.

Slyskova, J., Cordero, F., Pardini, B., Korenkova, V., Vymetalkova ,V., Bielik, L., Vodickova, L., Pitule, P., Liska, V., Matejka, V. M., Levy, M., Buchler, T., Kubista, M., Naccarati, A., Vodicka, P. Post-treatment recovery of suboptimal DNA repair capacity and gene expression levels in colorectal cancer patients. Molecular Carcinogenesis, 2014.

Vymetalkova, V. P., Slyskova, J., Korenkova ,V., Bielik, L., Langerova, L., Prochazka, P., Rejhova, A., Schwarzova, L., Pardini, B., Naccarati, A., Vodicka, P. Molecular characteristics of mismatch repair genes in sporadic colorectal tumors in Czech patients. BMC Med Genet., 15(1):17, 2014. doi: 10.1186/1471-2350-15-17. ISSN 1471-2350.

Zatecka, E., Ded, L., Elzeinova, F., Kubatova, A., Dorosh, A., Margaryan, H., Dostalova, P., Korenkova, V., Hoskova, K., Peknicova, J. Effect of zearalenone on reproductive parameters and expression of selected testicular genes in mice. Reproductive toxicology, 45: 20-30, 2014. doi:10.1016/j.reprotox.2014.01.003. ISSN 1873-1708.

Stahlberg, A., Kubista, M. The workflow of single-cell expression profiling using quantitative real-time PCR. Expert Review of Molecular Diagnostics, 14(3): 323–331, 2014. doi: 10.1586/14737159.2014.901154. ISSN 1473-7159.

Stahlberg, A., Aman, P., Strombom, L., Zoric, N., Diez, A., Nilsson, O., Kubista, M., Ridell, B. Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas. Hindawi Publishing Corporation, 2014: 1-6, 2014.

Sorg, G., Danowski, K., Korenkova, V., Rusnakova, V., Kueffner, R., Zimmer, R., Meyer, H. H. D., Kliem, H. Microfluidic high-throughput RT-qPCR measurements of the immune response of primary bovine mammary epithelial cells cultured from milk to mastitis pathogens. Animal, 7(5): 799-805, 2013. ISSN 1751-7311.

Bustin, S. A., Benes, V., Garson, J., Hellemans, J., Huggett, J., Kubista,... The need for transparency and good practices in the qPCR literature. Nature Methods, 10: 1063–1067, 2013. ISSN 1548-7091.

Svec, D., Andersson, D., Pekny M., Sjoback, R., Kubista, M., Stahlberg, A. Direct cell lysis for single-cell gene expression profiling. Front. Oncol, 3(274): 1-11, 2013.

Svec, D., Rusnakova, V., Korenkova, V., Kubista, M. Dye-based High-Throughput qPCR in Microfluidic Platform BioMark. In PCR Technology, Nolan, T. (ed.). CRC Press Inc., 323-339, 2013. ISBN 9781439848050.

Butenko, O., Benesova, J., Mikesova, M., Honsa, P., Dzamba, D., Kriska, J., Rusnakova, V., Kubista, M., Anderova, M. The fate of mdach1-expressing cells in the dorsal part of the lateral ventricles following focal cerebral ischemia. GLIA, 61(1): 207, 2013. ISSN 0894-1491.

Huggett, J. F., Foy, C. A., Benes, V., Emslie, K., Garson, J. A., Haynes, R., Hellemans, J., Kubista, M., Mueller, R. D., Nolan, T., Pfaffl, M. W., Shipley, G. L., Vandesompele, J., Bustin, S. A. The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments. Clinical Chemistry, 59(6): 892-902, 2013. ISSN 0009-9147.

Stahlberg, A, Rusnakova, V, Kubista, M. The added value of single-cell gene expression profiling. Brief Funct Genomics, 12(2): 81-89, 2013. ISSN 2041-2649.

Stahlberg, A., Rusnakova, V., Forootan, A., Anderova, M., Kubista, M. RT-qPCR work-flow for single-cell data analysis. Methods, 59(1): 80-88, 2013, ISSN 1046-2023.

Pazzagli, M., Malentacchi, F., Simi, L., Wyrich, R., Guenther, K., Hartmann, C., Verderio, P., Pizzamiglio, S., Ciniselli, C. M., Tichopad, A., Kubista, M., Gelmini, S. SPIDIA-RNA: First external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses. Methods, 59(1): 20-31, 2013. ISSN 1046-2023.

Stahlberg, A., Kubista, M. Unrevealing biological secrets by single cell expression profilig. Drug Discovery World Summer, 2013.