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Human oocyte maturation in vitro is improved by co-culture with cumulus cells from ...

Virant-Klun, I., Bauer, C., Stahlberg, A., Kubista, M., Skutella, T. Human oocyte maturation in vitro is improved by co-culture with cumulus cells from mature oocytes. Reproductive BioMedicine Online, 36(5): 508-523, 2018. doi: 10.1016/j.rbmo.2018.01.011. ISSN 1472-6483.



Molecular characterization of circulating tumor cells from patients with metastatic ...

Aaltonen, K. E., Novosadova, V., Bendahl, P., Graffman, C., Larsson, A., Ryden, L. Molecular characterization of circulating tumor cells from patients with metastatic breast cancer reflects evolutionary changes in gene expression under the pressure of systemic therapy. Oncotarget, 8:45544-45565, 2017. doi: 10.18632/oncotarget.17271. eISSN 1949-2553.



Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification

Androvic, P., Valihrach, L., Elling, J., Sjoback, R., Kubista, M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Research, gkx588, 2017. doi: 10.1093/nar/gkx588. ISSN 1362-4962.



Gene Expression Signatures in Circulating Tumor Cells Correlate with Response to ...

Bredemeier, M., Edimiris, P., Mach, P., Kubista, M., Sjoback, R., Rohlova, E., Kolostova, K., Hauch, S., Aktas, B., Tewes, M., Kimmig, R., Kasimir-Bauer, S. Gene Expression Signatures in Circulating Tumor Cells Correlate with Response to Therapy in Metastatic Breast Cancer. Clinical Chemistry, 63(10): 1585-1593, 2017. doi: 10.1373/clinchem.2016.269605. ISSN 0009-9147.



Horizontal transfer of whole mitochondria restores tumorigenic potential in ...

Dong, L. F., Kovarova, J., Bajzikova, M., Bezawork-Geleta, A., Svec, D.,... Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells. eLife, 6:e22187, 2017. doi: 10.7554/eLife.22187. ISSN 2050-084X.



Methods to determine limit of detection and limit of quantification in quantitative ...

Forootan, A., Sjoback, R., Bjorkman, J., Sjogreen, B., Linz, L., Kubista, M. Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR). Biomolecular Detection and Quantification, [ahead of print], 2017. doi: 10.1016/j.bdq.2017.04.001. ISSN 2214-7535.



Asymmetric Localization and Distribution of Factors Determining Cell Fate During Early ...

Sindelka, R., Sidova, M., Abaffy, P., Kubista, M. Asymmetric Localization and Distribution of Factors Determining Cell Fate During Early Development of Xenopus laevis. Chapter in Asymmetric Cell Division in Development, Differentiation and Cancer, Springer International Publishing, ed. J.-P. Tassan, J. Z. Kubiak, Results and Problems in Cell Differentiation, 61: 229-241, 2017. doi: 10.1007/978-3-319-53150-2_10. ISBN 978-3-319-53149-6.



The role of nitric oxide during embryonic epidermis development of Xenopus laevis

Tomankova, S., Abaffy, P., Sindelka, R. The role of nitric oxide during embryonic epidermis development of Xenopus laevis. Biology Open, bio.023739, 2017. doi: 10.1242/bio.023739. ISSN 2046-6390.



Regulatory mechanisms, expression levels and proliferation effects of the FUS-DDIT3 ...

Aman, P., Dolatabadi, S., Svec, D., Jonasson, E., Safavi, S., Andersson, D., Grundevik, P., Thomsen, C., Stahlberg, A. Regulatory mechanisms, expression levels and proliferation effects of the FUS-DDIT3 fusion oncogene in liposarcoma. Journal of Pathology, 238(5): 689-699, 2016. doi: 10.1002/path.4700. ISSN 1096-9896.



Differential amplicons (ΔAmp) - a new molecular method to assess RNA integrity

Bjorkman, J., Svec, D., Lott, E., Kubista, M., Sjoback, R. Differential amplicons (ΔAmp) - a new molecular method to assess RNA integrity. Biomolecular Detection and Quantification, Special Issue: Advanced Molecular Diagnostics for Biomaker Discovery - Part II(6): 4-12, 2016. doi: 10.1016/j.bdq.2015.09.002. ISSN 2214-7535.



Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and ...

Conrad, S., Azizi, H., Hatami, M., Kubista, M., Bonin, M., Hennenlotter, J., Slevert, K.-D., Skutella, T. Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells. Stem Cells International, 2016: 8582526, 2016. doi: 10.1155/2016/8582526.



The correlation between expression profiles measured in single cells and in ...

Dzamba, D., Valihrach, L., Kubista, M., Anderova, M. The correlation between expression profiles measured in single cells and in traditional bulk samples. Scientific Reports, 6: 37022 , 2016. doi: 10.1038/srep37022.



Accession of Tumor Heterogeneity by Multiplex Transcriptome Profiling of Single ...

Gorges, T. M., Kuske, A., Rock, K., Mauermann, O., Muller, V., Peine, S., Verpoort, K., Novosadova, V., Kubista, M., Riethdorf, S., Pantel, K. Accession of Tumor Heterogeneity by Multiplex Transcriptome Profiling of Single Circulating Tumor Cells. Clinical Chemistry, 62(11): 1504-1515, 2016. doi: 10.1373/clinchem.2016.260299. ISSN 0009-9147.



Gene expression profiling of circulating tumor cells and peripheral blood mononuclear ...

Hensler, M., Vancurova, I., Becht, E., Palata, O., Strnad, P., Tesarova, P., Cabinakova, M., Svec, D., Kubista, M., Bartunkova, J., Spisek, R., Sojka, L. Gene expression profiling of circulating tumor cells and peripheral blood mononuclear cells from breast cancer patients. OncoImmunology, 5(4): e1102827, 2016. doi: 10.1080/2162402X.2015.1102827. ISSN 2162-402X.



Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog

Honsa, P., Valny, M., Kriska, J., Matuskova, H., Harantova, L., Kirdajova, D., Valihrach, L., Androvic, P., Kubista, M., Anderova, M. Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog. GLIA, 64(9): 1518-1531, 2016. doi: 10.1002/glia.23019. ISSN 1098-1136.



The focus on sample quality: Influence of colon tissue collection on reliability of ...

Korenkova, V., Slyskova, J., Novosadova, V., Pizzamiglio, S., Langerova, L., Bjorkman, J., Vycital, O., Liska, V., Levy, M., Veskrna, K., Vodicka, P., Vodickova, L., Kubista, M., Verderio, P. The focus on sample quality: Influence of colon tissue collection on reliability of qPCR data. Scientific Reports, 6: 29023, 2016. doi: 10.1038/srep29023.



Identification and characterization of X. tropicalis common progenitors of Sertoli and ...

Tlapakova, T., Nguyen, T. M. X., Vegrichtova, M., Sidova, M., Strnadova, K., Blahova, M., Krylov, V. Identification and characterization of X. tropicalis common progenitors of Sertoli and peritubular myoid cell lineages. Biology Open, 019265, 2016. doi: 10.1242/bio.019265. eISSN 2046-6390.



MicroRNAs: From Female Fertility, Germ Cells, and Stem Cells to Cancer in Humans

Virant-Klun, I., Stahlberg, A., Kubista, M., Skutella, T. MicroRNAs: From Female Fertility, Germ Cells, and Stem Cells to Cancer in Humans. Stem Cells International, 2016: 3984937, 2016. doi: 10.1155/2016/3984937.



Properties of targeted preamplification in DNA and cDNA quantification

Andersson, D., Akrap, N., Svec, D., Godfrey, T. E., Kubista, M., Landberg, G., Stahlberg, A. Properties of targeted preamplification in DNA and cDNA quantification. Expert Review of Molecular Diagnostics, 15(8): 1085-1100, 2015. doi: 10.1586/14737159.2015.1057124. ISSN 1473-7159.



Quantitative Analysis of Glutamate Receptors in Glial Cells from the Cortex of ...

Dzamba, D., Honsa, P., Valny, M., Kriska, J., Valihrach, L., Novosadova, V., Kubista, M., Anderova, M. Quantitative Analysis of Glutamate Receptors in Glial Cells from the Cortex of GFAP/EGFP Mice Following Ischemic Injury: Focus on NMDA Receptors. Cellular and Molecular Neurobiology, 35(8): 1187-1202, 2015. doi: 10.1007/s10571-015-0212-8. ISSN 0272-434.



Urinary Biomarkers for Detection of Early and Advanced Chronic Kidney Disease ...

Korenkova, V., Jones, A., Hoy, W. E., Morais, Ch., Cooper, M. A., Gobe, G. C. Urinary Biomarkers for Detection of Early and Advanced Chronic Kidney Disease - A Pilot Study. Medicinal Chemistry, 5: 96-103, 2015. doi: 10.4172/2161-0444.1000249. ISSN 2161-04444.



Pre-amplification in the context of high-throughput qPCR gene expression experiment

Korenkova, V., Scott, J., Novosadova, V., Jindrichova, M., Langerova, L., Svec, D., Sidova, M., Sjoback, R. Pre-amplification in the context of high-throughput qPCR gene expression experiment. BMC Molecular Biology, 16: 5, 2015. doi: 10.1186/s12867-015-0033-9. ISSN 1471-2199.



Addressing biological heterogeneity with single cell profiling

Kubista, M. Addressing biological heterogeneity with single cell profiling. European Pharmaceutical Review, 20(3): In-depth focus: PCR, 2015.



Intracellular microRNA profiles form in the Xenopus laevis oocyte that may ...

Sidova, M., Sindelka, R., Castoldi, M., Benes, V., Kubista, M. Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division. Scientific Reports, 5: 11157, 2015. doi: 10.1038/srep11157. eISSN 2045-2322.



Effects of post-mortem and physical degradation on RNA integrity and quality

Sidova, M., Tomankova, S., Abaffy, P., Kubista, M., Sindelka, R. Effects of post-mortem and physical degradation on RNA integrity and quality. Biomolecular Detection and Quantification, 5: 3-9, 2015. doi: 10.1016/j.bdq.2015.08.002. ISSN 2214-7535.



How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR ...

Svec, D., Tichopad, A., Novosadova, V., Pfaffl, M. W., Kubista, M. How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments. Biomolecular Detection and Qualification, 3: 9-16, 2015. doi:10.1016/j.bdq.2015.01.005. ISSN 2214-7535.



Multi-template polymerase chain reaction

Kalle, E., Kubista, M., Rensing, Ch. Multi-template polymerase chain reaction. Biomolecular Detection and Qualification, 2: 11-29, 2014. doi:10.1016/j.bdq.2014.11.002. ISSN 2214-7535.



Altered Astrocytic Swelling in the Cortex of α-Syntrophin-Negative GFAP/EGFP Mice

Anderova, M., Benesova, J., Mikesova, M., Dzamba, D., Honsa, P., Kriska, J., Butenko, O., Novosadova, V., Valihrach, L., Kubista, M., Dmytrenko, L., Cicanic, M., Vargova, L. Altered Astrocytic Swelling in the Cortex of α-Syntrophin-Negative GFAP/EGFP Mice. PLoS ONE, 9(11): e113444, 2014. doi: 10.1371/journal.pone.0113444. ISSN 1932-6203.



Both genetic and dietary factors underlie individual differences in DNA damage levels ...

Slyskova, J., Lorenzo, Y., Karlsen, A., Carlsen, M. H., Novosadova, V., Blomhoff, R., Vodicka, P., Collins, A. R. Both genetic and dietary factors underlie individual differences in DNA damage levels and DNA repair capacity. DNA Repair, 16(2014): 66-73, 2014. doi: 10.1016/j.dnarep.2014.01.016. ISSN 1568-7864.



SPIDIA-RNA: Second External Quality Assessment for the Pre-Analytical Phase of Blood ...

Malentacchi, F., Pazzagli, M., Simi, M., Orlando, C., Wyrich, R., Gunther, K., Verderio, P., Pizzamiglio, S., Ciniselli, Ch. M., Zhang, H., Korenkova, V., Rainen, L., Bar, T., Kubista, M., Gelmini, S. SPIDIA-RNA: Second External Quality Assessment for the Pre-Analytical Phase of Blood Samples Used for RNA Based Analyses. PLoS ONE 9(11): e112293. doi:10.1371/journal.pone.0112293. ISSN 1932-6203.