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Gene expression profiling of circulating tumor cells and peripheral blood mononuclear ...

Hensler, M., Vancurova, I., Becht, E., Palata, O., Strnad, P., Tesarova, P., Cabinakova, M., Svec, D., Kubista, M., Bartunkova, J., Spisek, R., Sojka, L. Gene expression profiling of circulating tumor cells and peripheral blood mononuclear cells from breast cancer patients. OncoImmunology, 5(4): e1102827, 2016. doi: 10.1080/2162402X.2015.1102827. ISSN 2162-402X.



Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog

Honsa, P., Valny, M., Kriska, J., Matuskova, H., Harantova, L., Kirdajova, D., Valihrach, L., Androvic, P., Kubista, M., Anderova, M. Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog. GLIA, 64(9): 1518-1531, 2016. doi: 10.1002/glia.23019. ISSN 1098-1136.



The focus on sample quality: Influence of colon tissue collection on reliability of ...

Korenkova, V., Slyskova, J., Novosadova, V., Pizzamiglio, S., Langerova, L., Bjorkman, J., Vycital, O., Liska, V., Levy, M., Veskrna, K., Vodicka, P., Vodickova, L., Kubista, M., Verderio, P. The focus on sample quality: Influence of colon tissue collection on reliability of qPCR data. Scientific Reports, 6: 29023, 2016. doi: 10.1038/srep29023.



Identification and characterization of X. tropicalis common progenitors of Sertoli and ...

Tlapakova, T., Nguyen, T. M. X., Vegrichtova, M., Sidova, M., Strnadova, K., Blahova, M., Krylov, V. Identification and characterization of X. tropicalis common progenitors of Sertoli and peritubular myoid cell lineages. Biology Open, 019265, 2016. doi: 10.1242/bio.019265. eISSN 2046-6390.



MicroRNAs: From Female Fertility, Germ Cells, and Stem Cells to Cancer in Humans

Virant-Klun, I., Stahlberg, A., Kubista, M., Skutella, T. MicroRNAs: From Female Fertility, Germ Cells, and Stem Cells to Cancer in Humans. Stem Cells International, 2016: 3984937, 2016. doi: 10.1155/2016/3984937.



Properties of targeted preamplification in DNA and cDNA quantification

Andersson, D., Akrap, N., Svec, D., Godfrey, T. E., Kubista, M., Landberg, G., Stahlberg, A. Properties of targeted preamplification in DNA and cDNA quantification. Expert Review of Molecular Diagnostics, 15(8): 1085-1100, 2015. doi: 10.1586/14737159.2015.1057124. ISSN 1473-7159.



Quantitative Analysis of Glutamate Receptors in Glial Cells from the Cortex of ...

Dzamba, D., Honsa, P., Valny, M., Kriska, J., Valihrach, L., Novosadova, V., Kubista, M., Anderova, M. Quantitative Analysis of Glutamate Receptors in Glial Cells from the Cortex of GFAP/EGFP Mice Following Ischemic Injury: Focus on NMDA Receptors. Cellular and Molecular Neurobiology, 35(8): 1187-1202, 2015. doi: 10.1007/s10571-015-0212-8. ISSN 0272-434.



Urinary Biomarkers for Detection of Early and Advanced Chronic Kidney Disease ...

Korenkova, V., Jones, A., Hoy, W. E., Morais, Ch., Cooper, M. A., Gobe, G. C. Urinary Biomarkers for Detection of Early and Advanced Chronic Kidney Disease - A Pilot Study. Medicinal Chemistry, 5: 96-103, 2015. doi: 10.4172/2161-0444.1000249. ISSN 2161-04444.



Pre-amplification in the context of high-throughput qPCR gene expression experiment

Korenkova, V., Scott, J., Novosadova, V., Jindrichova, M., Langerova, L., Svec, D., Sidova, M., Sjoback, R. Pre-amplification in the context of high-throughput qPCR gene expression experiment. BMC Molecular Biology, 16: 5, 2015. doi: 10.1186/s12867-015-0033-9. ISSN 1471-2199.



Addressing biological heterogeneity with single cell profiling

Kubista, M. Addressing biological heterogeneity with single cell profiling. European Pharmaceutical Review, 20(3): In-depth focus: PCR, 2015.



Intracellular microRNA profiles form in the Xenopus laevis oocyte that may ...

Sidova, M., Sindelka, R., Castoldi, M., Benes, V., Kubista, M. Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division. Scientific Reports, 5: 11157, 2015. doi: 10.1038/srep11157. eISSN 2045-2322.



Effects of post-mortem and physical degradation on RNA integrity and quality

Sidova, M., Tomankova, S., Abaffy, P., Kubista, M., Sindelka, R. Effects of post-mortem and physical degradation on RNA integrity and quality. Biomolecular Detection and Quantification, 5: 3-9, 2015. doi: 10.1016/j.bdq.2015.08.002. ISSN 2214-7535.



How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR ...

Svec, D., Tichopad, A., Novosadova, V., Pfaffl, M. W., Kubista, M. How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments. Biomolecular Detection and Qualification, 3: 9-16, 2015. doi:10.1016/j.bdq.2015.01.005. ISSN 2214-7535.



Multi-template polymerase chain reaction

Kalle, E., Kubista, M., Rensing, Ch. Multi-template polymerase chain reaction. Biomolecular Detection and Qualification, 2: 11-29, 2014. doi:10.1016/j.bdq.2014.11.002. ISSN 2214-7535.



Altered Astrocytic Swelling in the Cortex of α-Syntrophin-Negative GFAP/EGFP Mice

Anderova, M., Benesova, J., Mikesova, M., Dzamba, D., Honsa, P., Kriska, J., Butenko, O., Novosadova, V., Valihrach, L., Kubista, M., Dmytrenko, L., Cicanic, M., Vargova, L. Altered Astrocytic Swelling in the Cortex of α-Syntrophin-Negative GFAP/EGFP Mice. PLoS ONE, 9(11): e113444, 2014. doi: 10.1371/journal.pone.0113444. ISSN 1932-6203.



Both genetic and dietary factors underlie individual differences in DNA damage levels ...

Slyskova, J., Lorenzo, Y., Karlsen, A., Carlsen, M. H., Novosadova, V., Blomhoff, R., Vodicka, P., Collins, A. R. Both genetic and dietary factors underlie individual differences in DNA damage levels and DNA repair capacity. DNA Repair, 16(2014): 66-73, 2014. doi: 10.1016/j.dnarep.2014.01.016. ISSN 1568-7864.



SPIDIA-RNA: Second External Quality Assessment for the Pre-Analytical Phase of Blood ...

Malentacchi, F., Pazzagli, M., Simi, M., Orlando, C., Wyrich, R., Gunther, K., Verderio, P., Pizzamiglio, S., Ciniselli, Ch. M., Zhang, H., Korenkova, V., Rainen, L., Bar, T., Kubista, M., Gelmini, S. SPIDIA-RNA: Second External Quality Assessment for the Pre-Analytical Phase of Blood Samples Used for RNA Based Analyses. PLoS ONE 9(11): e112293. doi:10.1371/journal.pone.0112293. ISSN 1932-6203.



Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples

Zhang, H., Korenkova, V., Sjoback, R., Svec, D., Bjorkman, J., Kruhoffer, M., Verderio, P., Pizzamiglio, S., Ciniselli, Ch. M., Wyrich, R., Oelmueller, U., Kubista, M., Lindahl, T., Lonneborg, A., Rian, E. Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples. PLoS ONE 9(11): e111644, 2014. doi:10.1371/journal.pone.0111644. ISSN 1932-6203.



The Extreme Anterior Domain Is an Essential Craniofacial Organizer Acting through ...

Jacox, L., Sindelka, R., Chen, J., Rothman, A., Dickinson, A., Sive, H. The Extreme Anterior Domain Is an Essential Craniofacial Organizer Acting through Kinin-Kallikrein Signaling. Cell Reports, 8(2): 596-609, 2014.



Prime time for qPCR – raising the quality bar

Kubista, M. Prime time for qPCR – raising the quality bar. European Pharmaceutical Review, 19(3): 63-67, 2014. 



High Throughput Few and Single Cell Expression Profiling and the Characterization of ...

Kubista, M., Sjoback, R., Jindrichova, M., Rohlova, E., Rusnakova, V., Hauch, S., Aktas, B., Tewes, M., Bredemeier, M., Kasimir-Bauer, S. High Throughput Few and Single Cell Expression Profiling and the Characterization of Circulating Tumor Cells. Anticancer Research, 34(10): 6014, 2014. Abstracts of the Ninth International Conference of Anticancer Research, October 6-10, 2014, Sithonia, Greece, Meeting Abstract: 350. ISSN 0250-7005.



Increased Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) ...

Honsa, P., Pivonkova, H., Harantova, L., Butenko, O., Kriska, J., Dzamba, D., Rusnakova, V., Valihrach, L., Kubista, M., Anderova, M. Increased Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in Reactive Astrocytes FollowingIschemia. GLIA, 62(12): 2004-2021, 2014. doi: 10.1002/glia.22721. ISSN 1098-1136.



Differential gene expression profiling of enriched human spermatogonia after short ...

Conrad, S., Azizi, H., Hatami, M., Kubista, M., Bonin, M., Hennenlotter, J., Renninger, M., Skutella, T. Differential gene expression profiling of enriched human spermatogonia after short- and long-term culture. Biomed Research International, 2014. Article Number: 138350, doi: 10.1155/2014/138350. ISSN 2314-6133. eISSN 2314-6141.



Post-treatment recovery of suboptimal DNA repair capacity and gene expression levels ....

Slyskova, J., Cordero, F., Pardini, B., Korenkova, V., Vymetalkova ,V., Bielik, L., Vodickova, L., Pitule, P., Liska, V., Matejka, V. M., Levy, M., Buchler, T., Kubista, M., Naccarati, A., Vodicka, P. Post-treatment recovery of suboptimal DNA repair capacity and gene expression levels in colorectal cancer patients. Molecular Carcinogenesis, 2014.



Molecular characteristics of mismatch repair genes in sporadic colorectal tumors in ...

Vymetalkova, V. P., Slyskova, J., Korenkova ,V., Bielik, L., Langerova, L., Prochazka, P., Rejhova, A., Schwarzova, L., Pardini, B., Naccarati, A., Vodicka, P. Molecular characteristics of mismatch repair genes in sporadic colorectal tumors in Czech patients. BMC Med Genet., 15(1):17, 2014. doi: 10.1186/1471-2350-15-17. ISSN 1471-2350.



Effect of zearalenone on reproductive parameters and expression of selected testicular ...

Zatecka, E., Ded, L., Elzeinova, F., Kubatova, A., Dorosh, A., Margaryan, H., Dostalova, P., Korenkova, V., Hoskova, K., Peknicova, J. Effect of zearalenone on reproductive parameters and expression of selected testicular genes in mice. Reproductive toxicology, 45: 20-30, 2014. doi:10.1016/j.reprotox.2014.01.003. ISSN 1873-1708.



The workflow of single-cell expression profiling using quantitative real-time PCR

Stahlberg, A., Kubista, M. The workflow of single-cell expression profiling using quantitative real-time PCR. Expert Review of Molecular Diagnostics, 14(3): 323–331, 2014. doi: 10.1586/14737159.2014.901154. ISSN 1473-7159.



Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and ...

Stahlberg, A., Aman, P., Strombom, L., Zoric, N., Diez, A., Nilsson, O., Kubista, M., Ridell, B. Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas. Hindawi Publishing Corporation, 2014: 1-6, 2014.



Microfluidic high-throughput RT-qPCR measurements of the immune response of primary ...

Sorg, G., Danowski, K., Korenkova, V., Rusnakova, V., Kueffner, R., Zimmer, R., Meyer, H. H. D., Kliem, H. Microfluidic high-throughput RT-qPCR measurements of the immune response of primary bovine mammary epithelial cells cultured from milk to mastitis pathogens. Animal, 7(5): 799-805, 2013. ISSN 1751-7311.



The need for transparency and good practices in the qPCR literature

Bustin, S. A., Benes, V., Garson, J., Hellemans, J., Huggett, J., Kubista,... The need for transparency and good practices in the qPCR literature. Nature Methods, 10: 1063–1067, 2013. ISSN 1548-7091.