Publications

Forootan, A., Sjoback, R., Bjorkman, J., Sjogreen, B., Linz, L., Kubista, M. Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR). Biomolecular Detection and Quantification, [ahead of print], 2017. doi: 10.1016/j.bdq.2017.04.001. ISSN 2214-7535.

Sindelka, R., Sidova, M., Abaffy, P., Kubista, M. Asymmetric Localization and Distribution of Factors Determining Cell Fate During Early Development of Xenopus laevis. Chapter in Asymmetric Cell Division in Development, Differentiation and Cancer, Springer International Publishing, ed. J.-P. Tassan, J. Z. Kubiak, Results and Problems in Cell Differentiation, 61: 229-241, 2017. doi: 10.1007/978-3-319-53150-2_10. ISBN 978-3-319-53149-6.

Tomankova, S., Abaffy, P., Sindelka, R. The role of nitric oxide during embryonic epidermis development of Xenopus laevis. Biology Open, bio.023739, 2017. doi: 10.1242/bio.023739. ISSN 2046-6390.

Aman, P., Dolatabadi, S., Svec, D., Jonasson, E., Safavi, S., Andersson, D., Grundevik, P., Thomsen, C., Stahlberg, A. Regulatory mechanisms, expression levels and proliferation effects of the FUS-DDIT3 fusion oncogene in liposarcoma. Journal of Pathology, 238(5): 689-699, 2016. doi: 10.1002/path.4700. ISSN 1096-9896.

Bjorkman, J., Svec, D., Lott, E., Kubista, M., Sjoback, R. Differential amplicons (ΔAmp) - a new molecular method to assess RNA integrity. Biomolecular Detection and Quantification, Special Issue: Advanced Molecular Diagnostics for Biomaker Discovery - Part II(6): 4-12, 2016. doi: 10.1016/j.bdq.2015.09.002. ISSN 2214-7535.

Conrad, S., Azizi, H., Hatami, M., Kubista, M., Bonin, M., Hennenlotter, J., Slevert, K.-D., Skutella, T. Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells. Stem Cells International, 2016: 8582526, 2016. doi: 10.1155/2016/8582526.

Dzamba, D., Valihrach, L., Kubista, M., Anderova, M. The correlation between expression profiles measured in single cells and in traditional bulk samples. Scientific Reports, 6: 37022 , 2016. doi: 10.1038/srep37022.

Gorges, T. M., Kuske, A., Rock, K., Mauermann, O., Muller, V., Peine, S., Verpoort, K., Novosadova, V., Kubista, M., Riethdorf, S., Pantel, K. Accession of Tumor Heterogeneity by Multiplex Transcriptome Profiling of Single Circulating Tumor Cells. Clinical Chemistry, 62(11): 1504-1515, 2016. doi: 10.1373/clinchem.2016.260299. ISSN 0009-9147.

Hensler, M., Vancurova, I., Becht, E., Palata, O., Strnad, P., Tesarova, P., Cabinakova, M., Svec, D., Kubista, M., Bartunkova, J., Spisek, R., Sojka, L. Gene expression profiling of circulating tumor cells and peripheral blood mononuclear cells from breast cancer patients. OncoImmunology, 5(4): e1102827, 2016. doi: 10.1080/2162402X.2015.1102827. ISSN 2162-402X.

Honsa, P., Valny, M., Kriska, J., Matuskova, H., Harantova, L., Kirdajova, D., Valihrach, L., Androvic, P., Kubista, M., Anderova, M. Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog. GLIA, 64(9): 1518-1531, 2016. doi: 10.1002/glia.23019. ISSN 1098-1136.

Korenkova, V., Slyskova, J., Novosadova, V., Pizzamiglio, S., Langerova, L., Bjorkman, J., Vycital, O., Liska, V., Levy, M., Veskrna, K., Vodicka, P., Vodickova, L., Kubista, M., Verderio, P. The focus on sample quality: Influence of colon tissue collection on reliability of qPCR data. Scientific Reports, 6: 29023, 2016. doi: 10.1038/srep29023.

Tlapakova, T., Nguyen, T. M. X., Vegrichtova, M., Sidova, M., Strnadova, K., Blahova, M., Krylov, V. Identification and characterization of X. tropicalis common progenitors of Sertoli and peritubular myoid cell lineages. Biology Open, 019265, 2016. doi: 10.1242/bio.019265. eISSN 2046-6390.

Virant-Klun, I., Stahlberg, A., Kubista, M., Skutella, T. MicroRNAs: From Female Fertility, Germ Cells, and Stem Cells to Cancer in Humans. Stem Cells International, 2016: 3984937, 2016. doi: 10.1155/2016/3984937.

Andersson, D., Akrap, N., Svec, D., Godfrey, T. E., Kubista, M., Landberg, G., Stahlberg, A. Properties of targeted preamplification in DNA and cDNA quantification. Expert Review of Molecular Diagnostics, 15(8): 1085-1100, 2015. doi: 10.1586/14737159.2015.1057124. ISSN 1473-7159.

Dzamba, D., Honsa, P., Valny, M., Kriska, J., Valihrach, L., Novosadova, V., Kubista, M., Anderova, M. Quantitative Analysis of Glutamate Receptors in Glial Cells from the Cortex of GFAP/EGFP Mice Following Ischemic Injury: Focus on NMDA Receptors. Cellular and Molecular Neurobiology, 35(8): 1187-1202, 2015. doi: 10.1007/s10571-015-0212-8. ISSN 0272-434.

Korenkova, V., Jones, A., Hoy, W. E., Morais, Ch., Cooper, M. A., Gobe, G. C. Urinary Biomarkers for Detection of Early and Advanced Chronic Kidney Disease - A Pilot Study. Medicinal Chemistry, 5: 96-103, 2015. doi: 10.4172/2161-0444.1000249. ISSN 2161-04444.

Korenkova, V., Scott, J., Novosadova, V., Jindrichova, M., Langerova, L., Svec, D., Sidova, M., Sjoback, R. Pre-amplification in the context of high-throughput qPCR gene expression experiment. BMC Molecular Biology, 16: 5, 2015. doi: 10.1186/s12867-015-0033-9. ISSN 1471-2199.

Kubista, M. Addressing biological heterogeneity with single cell profiling. European Pharmaceutical Review, 20(3): In-depth focus: PCR, 2015.

Sidova, M., Sindelka, R., Castoldi, M., Benes, V., Kubista, M. Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division. Scientific Reports, 5: 11157, 2015. doi: 10.1038/srep11157. eISSN 2045-2322.

Sidova, M., Tomankova, S., Abaffy, P., Kubista, M., Sindelka, R. Effects of post-mortem and physical degradation on RNA integrity and quality. Biomolecular Detection and Quantification, 5: 3-9, 2015. doi: 10.1016/j.bdq.2015.08.002. ISSN 2214-7535.

Svec, D., Tichopad, A., Novosadova, V., Pfaffl, M. W., Kubista, M. How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments. Biomolecular Detection and Qualification, 3: 9-16, 2015. doi:10.1016/j.bdq.2015.01.005. ISSN 2214-7535.

Kalle, E., Kubista, M., Rensing, Ch. Multi-template polymerase chain reaction. Biomolecular Detection and Qualification, 2: 11-29, 2014. doi:10.1016/j.bdq.2014.11.002. ISSN 2214-7535.

Anderova, M., Benesova, J., Mikesova, M., Dzamba, D., Honsa, P., Kriska, J., Butenko, O., Novosadova, V., Valihrach, L., Kubista, M., Dmytrenko, L., Cicanic, M., Vargova, L. Altered Astrocytic Swelling in the Cortex of α-Syntrophin-Negative GFAP/EGFP Mice. PLoS ONE, 9(11): e113444, 2014. doi: 10.1371/journal.pone.0113444. ISSN 1932-6203.

Slyskova, J., Lorenzo, Y., Karlsen, A., Carlsen, M. H., Novosadova, V., Blomhoff, R., Vodicka, P., Collins, A. R. Both genetic and dietary factors underlie individual differences in DNA damage levels and DNA repair capacity. DNA Repair, 16(2014): 66-73, 2014. doi: 10.1016/j.dnarep.2014.01.016. ISSN 1568-7864.

Malentacchi, F., Pazzagli, M., Simi, M., Orlando, C., Wyrich, R., Gunther, K., Verderio, P., Pizzamiglio, S., Ciniselli, Ch. M., Zhang, H., Korenkova, V., Rainen, L., Bar, T., Kubista, M., Gelmini, S. SPIDIA-RNA: Second External Quality Assessment for the Pre-Analytical Phase of Blood Samples Used for RNA Based Analyses. PLoS ONE 9(11): e112293. doi:10.1371/journal.pone.0112293. ISSN 1932-6203.

Zhang, H., Korenkova, V., Sjoback, R., Svec, D., Bjorkman, J., Kruhoffer, M., Verderio, P., Pizzamiglio, S., Ciniselli, Ch. M., Wyrich, R., Oelmueller, U., Kubista, M., Lindahl, T., Lonneborg, A., Rian, E. Biomarkers for Monitoring Pre-Analytical Quality Variation of mRNA in Blood Samples. PLoS ONE 9(11): e111644, 2014. doi:10.1371/journal.pone.0111644. ISSN 1932-6203.

Jacox, L., Sindelka, R., Chen, J., Rothman, A., Dickinson, A., Sive, H. The Extreme Anterior Domain Is an Essential Craniofacial Organizer Acting through Kinin-Kallikrein Signaling. Cell Reports, 8(2): 596-609, 2014.

Kubista, M. Prime time for qPCR – raising the quality bar. European Pharmaceutical Review, 19(3): 63-67, 2014. 

Kubista, M., Sjoback, R., Jindrichova, M., Rohlova, E., Rusnakova, V., Hauch, S., Aktas, B., Tewes, M., Bredemeier, M., Kasimir-Bauer, S. High Throughput Few and Single Cell Expression Profiling and the Characterization of Circulating Tumor Cells. Anticancer Research, 34(10): 6014, 2014. Abstracts of the Ninth International Conference of Anticancer Research, October 6-10, 2014, Sithonia, Greece, Meeting Abstract: 350. ISSN 0250-7005.

Honsa, P., Pivonkova, H., Harantova, L., Butenko, O., Kriska, J., Dzamba, D., Rusnakova, V., Valihrach, L., Kubista, M., Anderova, M. Increased Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in Reactive Astrocytes FollowingIschemia. GLIA, 62(12): 2004-2021, 2014. doi: 10.1002/glia.22721. ISSN 1098-1136.