Kubista, M. Prime time for qPCR – raising the quality bar. European Pharmaceutical Review, 19(3): 63-67, 2014.
Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids. Even though it has been around for more than 30 years and is preferred in research applications, it has still to win broad acceptance in routine. Main hurdles are the lack of guidelines, standards, quality controls, and even proper methods to evaluate the diagnostic results. This is now rapidly changing.