The Chromium Single Cell Gene Expression Solution provides a comprehensive, scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. Affordable and with a simplified workflow, users can go from cell sample to sequencing library in less than one workday. Single cells, reagents and a single Gel Bead containing barcoded oligonucleotides are encapsulated into nanoliter-sized GEMs (Gel Bead in emulsion) using the GemCode Technology. Lysis and barcoded reverse transcription of polyadenylated mRNA from single cells are performed inside each GEM. High-quality next generation sequencing libraries are finished in single bulk reaction. Finally, the Chromium™ Software Suite is utilized for processing, analysis and visualization of single cell gene expression data.
Laboratory of Gene Expression
Institute of Biotechnology CAS, v.v.i.
Prumyslova 595
252 50 Vestec
Czech Republic
Phone: +420 325 873 748, +420 325 873 747