The Chromium Single Cell Gene Expression Solution provides a comprehensive, scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. Affordable and with a simplified workflow, users can go from cell sample to sequencing library in less than one workday. Single cells, reagents and a single Gel Bead containing barcoded oligonucleotides are encapsulated into nanoliter-sized GEMs (Gel Bead in emulsion) using the GemCode Technology. Lysis and barcoded reverse transcription of polyadenylated mRNA from single cells are performed inside each GEM. High-quality next generation sequencing libraries are finished in single bulk reaction. Finally, the Chromium™ Software Suite is utilized for processing, analysis and visualization of single cell gene expression data.
Laboratory of Gene Expression
Institute of Biotechnology CAS, v.v.i.
252 50 Vestec
Phone: +420 325 873 748, +420 325 873 747